PXD053676

PXD053676 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Wilms tumor-associated ENL mutants promote MOZ/MORF-mediated DOT1L loading in kidney tumor and leukemia
Description	ENL is mutated in both Wilms tumor and leukemia which are the two major types of childhood cancers that occur at an early age. This study demonstrates that ENL mutants promote the loading of the DOT1L complex onto promoters in a MOZ/MORF histone acetyltransferase- dependent manner. The ENL protein is an epigenetic hub that interacts with multiple functionally distinct protein complexes to regulate transcription. MOZ binds to ENL through its YEATS domain to form a MOZ/ENL complex, which further recruits a DOT1L/AF10 complex and loads the ENL/DOT1L/AF10 complex onto nearby chromatin. Small indels in the YEATS domain increase the affinity to MOZ/MORF, which potentiates the interaction with ENL and the loading of the DOT1L complex, thereby hyperactivating HOX gene expression in embryonic kidney cells and hematopoietic cells. This oncogenic transformation can be suppressed by inhibitors of the histone-modifying activities of MOZ/MORF and DOT1L, paving the way for molecular targeted therapies against ENL-mutated cancers.
HostingRepository	jPOST
AnnounceDate	2025-07-05
AnnouncementXML	Submission_2025-07-04_08:00:06.108.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Takeshi Kawamura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 00:15:08	ID requested
⏵ 1	2025-07-04 08:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Wilms tumor
Leukemia
lysine acyl transferase
lysine methyltransferase
ENL
MOZ
DOT1L
HOX

submitter keyword: Wilms tumor

Leukemia

lysine acyl transferase

lysine methyltransferase

ENL

MOZ

DOT1L

HOX

Contact List

Akihiko Yokoyama
lab head
Takeshi Kawamura
contact affiliation	Tokyo University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003201/