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PXD053646

PXD053646 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular Basis of Spindle Morphology Changes at the Meiosis to Mitosis Transition
DescriptionThe transition from meiotic divisions in the oocyte to mitotic divisions in the early embryo is a critical step in animal development. Despite negligible changes to cell size, shape, and content, following fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle that nucleates abundant astral microtubules at spindle poles. To identify underlying mechanisms, we applied a drug screening approach using eggs of the simple chordate Ciona robusta (sea squirt) and found that inhibition of Casein Kinase 2 (CK2) caused a shift from meiotic to mitotic-like spindle morphology with nucleation of robust astral microtubule arrays, an effect reproduced in Xenopus laevis (frog) egg extracts. In both species, a kinase assay revealed that CK2 activity decreases at fertilization. To identify substrates and downstream effectors, we assessed the global phosphoproteomic changes that accompany both the meiosis to mitosis transition and CK2 inhibition in Xenopus, which identified components of the Ran-GTP pathway as potential downstream targets. Inhibition of Ran-GTP-driven microtubule nucleation suppressed CK2-induced spindle morphology changes in both Xenopus and Ciona. These data support a model in which attenuation of CK2 activity at fertilization in chordates leads to activation of Ran-regulated spindle assembly factors that drive microtubule growth and the transition to mitotic spindle morphology.
HostingRepositoryMassIVE
AnnounceDate2025-02-02
AnnouncementXMLSubmission_2025-02-02_09:27:43.309.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterArminja Nadine Kettenbach
SpeciesList scientific name: Xenopus laevis; common name: African clawed frog; NCBI TaxID: 8355;
ModificationListPhospho
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-04 12:13:36ID requested
12025-02-02 09:27:43announced
Publication List
no publication
Keyword List
submitter keyword: Frog, mitosis, meiosis, embryo, cell cycle phases
Contact List
Arminja Nadine Kettenbach
contact affiliationDartmouth College
contact emailArminja.N.Kettenbach@Dartmouth.edu
lab head
Arminja Nadine Kettenbach
contact affiliationDartmouth
contact emailArminja.N.Kettenbach@Dartmouth.edu
dataset submitter
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MassIVE dataset URI
Dataset FTP location
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