PXD053646

PXD053646 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Molecular Basis of Spindle Morphology Changes at the Meiosis to Mitosis Transition
Description	The transition from meiotic divisions in the oocyte to mitotic divisions in the early embryo is a critical step in animal development. Despite negligible changes to cell size, shape, and content, following fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle that nucleates abundant astral microtubules at spindle poles. To identify underlying mechanisms, we applied a drug screening approach using eggs of the simple chordate Ciona robusta (sea squirt) and found that inhibition of Casein Kinase 2 (CK2) caused a shift from meiotic to mitotic-like spindle morphology with nucleation of robust astral microtubule arrays, an effect reproduced in Xenopus laevis (frog) egg extracts. In both species, a kinase assay revealed that CK2 activity decreases at fertilization. To identify substrates and downstream effectors, we assessed the global phosphoproteomic changes that accompany both the meiosis to mitosis transition and CK2 inhibition in Xenopus, which identified components of the Ran-GTP pathway as potential downstream targets. Inhibition of Ran-GTP-driven microtubule nucleation suppressed CK2-induced spindle morphology changes in both Xenopus and Ciona. These data support a model in which attenuation of CK2 activity at fertilization in chordates leads to activation of Ran-regulated spindle assembly factors that drive microtubule growth and the transition to mitotic spindle morphology.
HostingRepository	MassIVE
AnnounceDate	2025-02-02
AnnouncementXML	Submission_2025-02-02_09:27:43.309.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arminja Nadine Kettenbach
SpeciesList	scientific name: Xenopus laevis; common name: African clawed frog; NCBI TaxID: 8355;
ModificationList	Phospho
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-04 12:13:36	ID requested
⏵ 1	2025-02-02 09:27:43	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Frog, mitosis, meiosis, embryo, cell cycle phases

submitter keyword: Frog, mitosis, meiosis, embryo, cell cycle phases

Contact List

Arminja Nadine Kettenbach
contact affiliation	Dartmouth College
contact email	Arminja.N.Kettenbach@Dartmouth.edu
lab head
Arminja Nadine Kettenbach
contact affiliation	Dartmouth
contact email	Arminja.N.Kettenbach@Dartmouth.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095250/