PXD053646
PXD053646 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular Basis of Spindle Morphology Changes at the Meiosis to Mitosis Transition |
Description | The transition from meiotic divisions in the oocyte to mitotic divisions in the early embryo is a critical step in animal development. Despite negligible changes to cell size, shape, and content, following fertilization the small, barrel-shaped meiotic spindle is replaced by a large zygotic spindle that nucleates abundant astral microtubules at spindle poles. To identify underlying mechanisms, we applied a drug screening approach using eggs of the simple chordate Ciona robusta (sea squirt) and found that inhibition of Casein Kinase 2 (CK2) caused a shift from meiotic to mitotic-like spindle morphology with nucleation of robust astral microtubule arrays, an effect reproduced in Xenopus laevis (frog) egg extracts. In both species, a kinase assay revealed that CK2 activity decreases at fertilization. To identify substrates and downstream effectors, we assessed the global phosphoproteomic changes that accompany both the meiosis to mitosis transition and CK2 inhibition in Xenopus, which identified components of the Ran-GTP pathway as potential downstream targets. Inhibition of Ran-GTP-driven microtubule nucleation suppressed CK2-induced spindle morphology changes in both Xenopus and Ciona. These data support a model in which attenuation of CK2 activity at fertilization in chordates leads to activation of Ran-regulated spindle assembly factors that drive microtubule growth and the transition to mitotic spindle morphology. |
HostingRepository | MassIVE |
AnnounceDate | 2025-02-02 |
AnnouncementXML | Submission_2025-02-02_09:27:43.309.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Arminja Nadine Kettenbach |
SpeciesList | scientific name: Xenopus laevis; common name: African clawed frog; NCBI TaxID: 8355; |
ModificationList | Phospho |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-07-04 12:13:36 | ID requested | |
⏵ 1 | 2025-02-02 09:27:43 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Frog, mitosis, meiosis, embryo, cell cycle phases |
Contact List
Arminja Nadine Kettenbach | |
---|---|
contact affiliation | Dartmouth College |
contact email | Arminja.N.Kettenbach@Dartmouth.edu |
lab head | |
Arminja Nadine Kettenbach | |
contact affiliation | Dartmouth |
contact email | Arminja.N.Kettenbach@Dartmouth.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095250/ |