PXD053642

PXD053642 is an original dataset announced via ProteomeXchange.

Title	Molecular structure of blue laccase BP76, a unique termite suicidal defense weapon
Description	Aging workers of the termite Neocapritermes taracua can defend their colony by sacrificing themselves by body rupture, mixing the externally stored blue laccase BP76 with hydroquinones to produce a sticky liquid rich in toxic benzoquinones. The study elucidates structure of BP76 isolated from N. taracua in its native form. The MS part of the study focused on characterization of the modification product of a metastable crosslink between K189 and C292, and to confirm suspected mutations. The remarkable stability of BP76 maintains its catalytic activity in solid state during the lifespan of N. taracua workers, providing old workers with an efficient defensive weapon to protect their colony. This work was supported by The Ministry of Education, Youth and Sports of the Czech Republic grant PHOTOMACHINES - Photosynthetic cell redesign for high yields of therapeutic peptides (CZ.02.01.01/00/22_008/0004624) and the Structural Mass Spectrometry Core Facility of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2018127) and the European Regional Development Fund Project, UP "CIISB" (No. CZ.02.1.01/0.0/0.0/18_046/0015974).
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_05:26:45.616.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053642
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marek Zakopcanik
SpeciesList	scientific name: Neocapritermes taracua; NCBI TaxID: 377960;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-04 06:12:54	ID requested
⏵ 1	2025-03-13 05:26:46	announced

10.6019/PXD053642;

10.1016/J.STR.2024.07.015;

submitter keyword: benzoquinone, insect glycosylation, protein stabilization, laccase, colony defence, mass spectrometry, autothysis,Neocapritermes taracua, timsToF SCP

Petr Novak
contact affiliation	Laboratory of structural biology and cell signaling Institute of Microbiology Videnska 1083, 142 20 Prague Czech Republic
contact email	pnovak@biomed.cas.cz
lab head
Marek Zakopcanik
contact affiliation	Laboratory of structural biology and cell signaling, Institute of Microbiology, Czech Academy of Sciences, v.v.i., Videnska 1083, 142 20 Prague Czech Republic
contact email	marek.zakopcanik@biomed.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053642
     1. Label: PRIDE project
     2. Name: Molecular structure of blue laccase BP76, a unique termite suicidal defense weapon