PXD053626

PXD053626 is an original dataset announced via ProteomeXchange.

Title	GlycoPCT: quantitative glycoproteomics based on pressure cycling technology reveals distinctive N-glycosylation in human liver with non-alcoholic fatty liver disease
Description	Protein N-glycosylation plays a crucial role in the human liver, impacting key functions like hepatocyte lipid metabolism hepatocyte apoptosis, and inflammatory response. Despite its significance, the site-specific N-glycosylation patterns and their variations of liver biosy samples between healthy individuals and those with non-alcoholic fatty liver disease (NAFLD) have not been fully revealed. To address this issue, we presented a quantitative glycoproteomics called GlycoPCT based on pressure cycling technology. This method allows for the efficient recovery of intact N-glycopeptides (IGPs) and provides a thorough and accurate quantitative analysis of trace liver biopsy samples. Our research uncovered a total of 4,459 unique IGPs and 361 glycans from 758 glycoproteins. Remarkably, we identified 182 upregulated IGPs from 67 proteins (p<0.05, FC>1.50) and 108 downregulated IGPs from 44 proteins (p<0.05, FC<0.67) in the NAFLD group. Among these, we highlighted an essential acute phase glycoprotein, alpha-1-acid glycoprotein 1 (A1TA), which is synthesized in the liver and plays a significant role in the NAFLD progression. Besides, the complex-type, fucosylation-type and sialylation-type N-glycans were upregulated significantly in NAFLD group (p<0.001, t-test). These differentially expressed N-glycosylation modifications provide important clues for the diagnosis and pathogenesis of NAFLD.
HostingRepository	iProX
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-12-16_23:10:28.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 20:41:20	ID requested
⏵ 1	2024-12-16 23:10:29	announced

Jiang W, Liu M, Su T, Jin Y, Ling Y, Liu CH, Tang H, Wu D, Zhang Y, GlycoPCT: Pressure Cycling Technology-Based Quantitative Glycoproteomics Reveals Distinctive N-Glycosylation in Human Liver Biopsy Samples of Nonalcoholic Fatty Liver Disease. J Proteome Res, 24(1):202-209(2025) [pubmed]

submitter keyword: Glycoproteomics, N-glycosylation, Pressure cycling technology, Non-alcoholic fatty liver disease, LC-MS/MS.

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI