PXD053613

PXD053613 is an original dataset announced via ProteomeXchange.

Title	Mouse testis Co-IP LC-MSMS for studying HSF5 function
Description	The pachynema progression, a crucial meiotic process, contributes to the completion of prophase I. Nevertheless, the regulation of this significant meiotic process remains poorly understood. In this study, we identified a novel testis-specific protein HSF5, which regulates pachynema progression during male meiosis in a chromatin-binding manner. Deficiency of HSF5 results in meiotic arrest and male infertility, characterized as unconventionally accumulated pachynema halting at the mid-to-late stage, with extensive spermatocyte apoptosis. Our scRNA-seq data confirmed consistent expressional alterations of certain driver genes(Sycp1, Msh4, Meiob, etc.) crucial for pachynema progression in Hsf5-/- individuals. Ulteriorly, HSF5 was revealed to primarily bind to promoter regions of such key divers by CUT&Tag analysis. Also, our results demonstrated that HSF5 biologically interacted with the chromatin-remodeling SWI/SNF complex, and it could function as a transcriptional factor for pachynema progression during meiosis. Therefore, our study underscored the importance of the chromatin-associated HSF5 for the differentiation of spermatocytes, improving the protein regulatory network of the pachynema progression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:09.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ziqi Yu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 13:46:21	ID requested
1	2024-08-23 05:30:19	announced
⏵ 2	2024-10-22 06:56:09	announced	2024-10-22: Updated project metadata.

10.1093/NAR/GKAE701;

submitter keyword: LC-MSMS,Mouse, Co-IP, HSF5, Testis

Fei Sun
contact affiliation	Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, China.
contact email	sunfeidrrsh@zju.edu.cn
lab head
ziqi Yu
contact affiliation	Department of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
contact email	mr.yuziqi@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053613
     1. Label: PRIDE project
     2. Name: Mouse testis Co-IP LC-MSMS for studying HSF5 function