PXD053609

PXD053609 is an original dataset announced via ProteomeXchange.

Title	Partners of different NHSL3 isoforms
Description	MCF10A cells were genome edited to tag the endogenous ORF of NHSL3 and generate C-terminal GFP-Flag fusion proteins of all NHSL3 isoforms. MCF10A cells express 4 NHSL3 isoforms through the use of alternative exons (i1, i2, i3L and i3S). Plasmids encoding N-terminal Flag-GFP fusion proteins were constructed and used to select stable MCF10A cell lines expressing each of the tagged isoform. The 4 distinct NHSL3 isoforms in these stable cell lines as well as all isoforms together from the genome edited cell line were purified by Tandem Affinity Purification and associated proteins were identified by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-04_05:02:16.640.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Chiappetta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 08:50:28	ID requested
⏵ 1	2025-03-04 05:02:17	announced

10.1038/S41467-024-55647-3;

submitter keyword: human

Joelle Vinh
contact affiliation	UMR8249 - SMBP, Mass Spectrometry Biology and Proteomic, ESPCI – CNRS
contact email	joelle.vinh@espci.fr
lab head
Giovanni Chiappetta
contact affiliation	ESPCI-CNRS
contact email	giovanni.chiappetta@espci.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD053609

PRIDE project URI

• PRIDE
  1. PXD053609
     1. Label: PRIDE project
     2. Name: Partners of different NHSL3 isoforms