PXD053591 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TurboID-mediated proximity labeling of DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans |
| Description | TurboID-mediated proximity labeling of the aging and stress regulatory transcription factor DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans. Endogenous DAF-16 was C-terminally tagged with the biotin ligase TurboID using CRISPR/Cas9- genome editing. In vivo proximity labeling of the DAF-16 interactome was analyzed at normal and low insulin/IGF-1 signaling conditions. The biotin-labeled DAF-16 interactome was affinity purified using streptavidin resin and analyzed with mass spec. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-28_16:16:46.522.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Markus Hartl |
| SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239; |
| ModificationList | biotinylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-07-03 00:46:38 | ID requested | |
| ⏵ 1 | 2025-12-28 16:16:47 | announced | |
Publication List
| 10.1038/s41467-025-66409-0; |
| Artan M, Schoen H, de Bono M, Proximity labeling of DAF-16 FOXO highlights aging regulatory proteins. Nat Commun, 16(1):11355(2025) [pubmed] |
Keyword List
| submitter keyword: FOXO, TurboID, insulin / IGF signaling, DAF-16, Caenorhabditis elegans,Proximity labeling |
Contact List
| Mario de Bono |
|---|
| contact affiliation | Institute of Science and Technology Austria (ISTA) |
| contact email | Mario.deBono@ist.ac.at |
| lab head | |
| Markus Hartl |
|---|
| contact affiliation | Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria |
| contact email | proteomics@maxperutzlabs.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053591
- Label: PRIDE project
- Name: TurboID-mediated proximity labeling of DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans
