PXD053580

PXD053580 is an original dataset announced via ProteomeXchange.

Title	Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth
Description	Acetyl-CoA carboxylase (ACCase) catalyzes the first and rate-limiting step in de novo fatty acid synthesis in the chloroplast. ACCase activity has thus been subjected to positive and negative regulations. Among negative regulators, one finds the pII, BADC and the carboxyltransferase interactor (CTI) in Arabidopsis thaliana. However, we know little about the regulation of fatty acid synthesis in algae. In this study, we identified only one homolog of the plant CTI in Chlamydomonas and show that the Chlamydomonas CrCTI1 indeed interacts with the Chlamydomonas alhpa-carboxyltransferase (CT) ACCase subunit by yeast two-hybrid protein-protein interaction assay. The Chlamydomonas CrCTI1 knocked-out mutants, obtained from the CRISPR-Cas9 mutagenesis, accumulated higher amount of total fatty acids and stored more triacylglycerols (TAGs) in lipid droplets, without affecting membrane lipids. The TAG phenotype of the crcti1 mutants was not influenced by light, but rather is affected by trophic styles. Moreover, cell cultivation under heterotrophic conditions in darkness revealed the crucial function of CrCTI1 in homeostasis between lipid accumulation and cell growth. Comparative proteomics analysis of the crcti1 mutants and WT suggested a role for CrCTI1 in maintaining carbon flux and redox balance under different carbon availability. Taken together, this study reveals the occurrence and function of a negative regulator of fatty acid synthesis in algae and provides a molecular brick that can be used for genetic engineering of microalgae for biotechnology purposes.
HostingRepository	PRIDE
AnnounceDate	2025-02-26
AnnouncementXML	Submission_2025-02-26_07:05:31.413.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Lemes Jorge
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-02 15:45:55	ID requested
⏵ 1	2025-02-26 07:05:31	announced

10.1111/PBI.14581;

submitter keyword: acetyl-CoA carboxylase

carboxyltransferase interactor

de novo fatty acid synthesis

ROS

peroxisome fatty acid β-oxidation

cell growth

Yonghua Li-Beisson
contact affiliation	Aix-Marseille Université, CEA, CNRS, Institute of Biosciences and Biotechnologies of Aix-Marseille, UMR 7265, CEA Cadarache, Saint-Paul-lez Durance F-13108, France
contact email	yonghua.li@cea.fr
lab head
Gabriel Lemes Jorge
contact affiliation	University of Missouri
contact email	glj2d5@umsystem.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053580

PRIDE project URI

• PRIDE
  1. PXD053580
     1. Label: PRIDE project
     2. Name: Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth