PXD053568

PXD053568 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of CSF from the Alzheimer's disease mouse model 5xFAD
Description	The 5xFAD mouse model was used to study the influence of amyloid pathology on the cerebrospinal fluid (CSF) proteome. Therefore, CSF of 7-months old 5xFAD and WT control mice was sampled with an improved collection protocol. Using a minute amount of 5 µL CSF, we applied data dependent and data independent acquisition of the same samples on a Q-Exactive HF mass spectrometer. Comparison of DDA and DIA demonstrate a higher data completeness for DIA. The results show a strong effect on microglia activation exemplified by strongly increased abundances of Trem2 and Cst7. Furthermore, neurodegenerative markers such as neurofilaments and tau (Mapt) were strongly increased for 5xFAD mice.
HostingRepository	PRIDE
AnnounceDate	2025-04-15
AnnouncementXML	Submission_2025-04-15_09:14:09.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-02 08:16:16	ID requested
⏵ 1	2025-04-15 09:14:10	announced

Lourbopoulos A, M, ü, ller SA, Jocher G, Wick M, Plesnila N, Lichtenthaler SF, An Improved Method for Sampling and Quantitative Protein Analytics of Cerebrospinal Fluid of Individual Mice. Mol Cell Proteomics, 24(5):100958(2025) [pubmed]

10.1016/j.mcpro.2025.100958;

submitter keyword: cerebrospinal fluid, DIA,5xFAD, Alzheimer's disease, CSF, DDA

Stefan Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053568
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of CSF from the Alzheimer's disease mouse model 5xFAD