PXD053532

PXD053532 is an original dataset announced via ProteomeXchange.

Title	AVATARS - Tissue-specific multi-omics analyses in developing Brassica napus seeds [proteomics]
Description	Seeds of the German winter-type oilseed rape cultivar Express 617 were sampled and analysed at five key stages of seed development. Express 617 is a high oil yield cultivar with double-zero quality (low erucic acid and low glucosinolate content), which has been widely used in breeding programs and as parent for the creation of genetic mapping populations. Plants were grown under controlled, field-like conditions in the container-based system of the IPK PhenoSphere (Heuermann et al., 2023). The weather regime of the cultivation (2021/2021) was based on twelve years of hourly records of the weather station in Gatersleben and was designed to be as representative as possible of a typical growth season. Avoiding stressful weather extremes, the cultivation regime was considered to be favourable for plant growth and seed formation. Siliques were sampled at five key stages of seed development, as outlined by Borisjuk et al. (2013), covering the development from the pre-storage stage to seed maturation. To provide spatial information, seeds were manually dissected into four organs/tissues: the seed coat (SC), the inner (IC) and outer cotyledon (OC), and the radicle (RA). Aliquots of the pooled materials were subjected to mRNA-Sequencing and spectrometry–based proteomic analyses. Samples were prepared via the SP3 protocol and peptides were analysed on a timsTOF Pro mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2025-03-19
AnnouncementXML	Submission_2025-03-19_02:51:58.991.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053532
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nils Rugen
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-01 13:34:18	ID requested
⏵ 1	2025-03-19 02:51:59	announced

10.6019/PXD053532;

submitter keyword: seed filling, seed coat, rapeseed,brassica napus, seeds

seed development, cotyledons, radicle

Hans-Peter Braun
contact affiliation	Leibniz University Hannover Institute of Plant Genetics Section Plant Molecular Biology and Plant Proteomics
contact email	braun@genetik.uni-hannover.de
lab head
Nils Rugen
contact affiliation	Institut für Pflanzengenetik Leibniz Universität Hannover Herrenhäuser Str. 2 30419 Hannover Germany
contact email	rugen@genetik.uni-hannover.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053532
     1. Label: PRIDE project
     2. Name: AVATARS - Tissue-specific multi-omics analyses in developing Brassica napus seeds [proteomics]