PXD053509

PXD053509 is an original dataset announced via ProteomeXchange.

Title	EXPLORING THE INTERACTOME OF PML NUCLEAR SUBDOMAINS DURING FATTY ACID STRESS USING APEX2-MEDIATED PROXIMITY LABELING
Description	When exposed to excess fatty acids, specific cell types produce nuclear lipid droplets (nLDs) that associate with promyelocytic leukemia (PML) protein to form Lipid Associated PML Structures (LAPS) that are enriched in lipid biosynthetic enzymes but deficient in canonical proteins associated with PML nuclear bodies (PML NBs). To identify the PML interactome during lipid stress, we employed proximity-dependent biotin identification (BioID) in U2OS cells expressing PMLI and PMLII fused to the ascorbate peroxidase APEX2 and cultured in the absence or presence of oleate to enhance lipid droplet formation. The resulting interactome included proteins enriched under oleate-treated conditions, such mitogen activated protein kinase-activated protein kinase 2 (MK2), ESCRT proteins and the COPII vesicle transport proteins Sec23B, Sec24A and USO1. COPII proteins co-localized with both PML-NBs and LAPS but were selectively enriched in PML-NBs following oleate treatment. The nuclear localization of USO1 was uniquely dependent on PML expression. Thus, the APEX2-PML proximity interactome implicates PML domains in the nuclear function of a non-canonical network of COPII vesicle trafficking proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_21:22:15.408.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Levesque
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-29 06:11:21	ID requested
⏵ 1	2025-05-06 21:22:16	announced

10.1002/1873-3468.15081;

Thompson J, Boisvert FM, Salsman J, L, é, vesque D, Dellaire G, Ridgway ND, The proximity interactome of PML isoforms I and II under fatty acid stress. FEBS Lett, 599(5):682-699(2025) [pubmed]

submitter keyword: Nuclear Lipid Droplet, Promyelocytic Leukemia, COPII, Proximity-Dependent Biotin Identification,APEX2, Lipid-Associated PML Structures

Neale D. Ridgway
contact affiliation	Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Nova Scotia Canada B3H4R2, Department of Pediatrics, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia Canada B3H4R2
contact email	nridgway@dal.ca
lab head
Dominique Levesque
contact affiliation	University of Sherbrooke, FM Boisvert lab
contact email	dominique.levesque@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053509

PRIDE project URI

• PRIDE
  1. PXD053509
     1. Label: PRIDE project
     2. Name: EXPLORING THE INTERACTOME OF PML NUCLEAR SUBDOMAINS DURING FATTY ACID STRESS USING APEX2-MEDIATED PROXIMITY LABELING