PXD053493

PXD053493 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The PNUTS phosphatase complex controls transcription pause release
Description	Gene expression is regulated by controlling distinct steps of the transcriptional cycle, including initiation, pausing, elongation, and termination. Kinases phosphorylate RNA Polymerase II and associated factors to control transitions between these steps and act as central gene regulatory nodes. Similarly, phosphatases that dephosphorylate these components are emerging as important regulators of transcription, though their roles remain less well understood. Here we discover that the PNUTS-PP1 phosphatase complex plays an essential role in controlling transcription pause release in addition to its previously described function in transcription termination. Transcription pause release by the PNUTS complex is essential for almost all RNA Pol II-dependent gene transcription, relies on its PP1 phosphatase subunit, and controls the phosphorylation of factors required for pause release and elongation. Together, this reveals an essential new role for a phosphatase complex in transcription pause release and shows that the PNUTS complex is essential for RNA Poll II-dependent transcription.
HostingRepository	MassIVE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_06:28:50.214.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Arminja Nadine Kettenbach
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Phospho
Instrument	Orbitrap Fusion Lumos; Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-28 09:35:18	ID requested
⏵ 1	2024-10-07 06:28:50	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: PP1, PNUTS, transcription, phosphorylation, degron

submitter keyword: PP1, PNUTS, transcription, phosphorylation, degron

Contact List

Arminja Nadine Kettenbach
contact affiliation	Dartmouth
contact email	Arminja.N.Kettenbach@Dartmouth.edu
lab head
Arminja Nadine Kettenbach
contact affiliation	Dartmouth
contact email	Arminja.N.Kettenbach@Dartmouth.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095177/