PXD053490

PXD053490 is an original dataset announced via ProteomeXchange.

Title	Downregulation of Human-Specific lncRNA TMEM9B-AS1 in Skeletal Muscle of People with Type 2 Diabetes Impacts Ribosomal Biogenesis
Description	Long non-coding RNAs (lncRNAs) are important regulators of skeletal muscle physiology, with altered expression noted in several human diseases including type 2 diabetes. Here we report downregulation of TMEM9B-AS1, a previously uncharacterized lncRNA, in skeletal muscle of men with type 2 diabetes. We demonstrate that loss of TMEM9B-AS1 in primary human myotubes leads to reduction in protein synthesis, concomitant with reduced phosphorylation of ribosomal protein S6, downstream of ERK and mTOR pathways. Moreover, we show that TMEM9B-AS1 plays a pivotal role in the regulation of ribosomal biogenesis by facilitating mRNA stabilization of the transcription factor MYC through a direct physical interaction with the RNA-binding protein IGF2BP1. Disrupted ribosomal biogenesis resulting from downregulation of TMEM9B-AS1 links to the decrease in skeletal muscle mass observed in individuals with type 2 diabetes, shedding new light on the molecular mechanisms underlying this metabolic disorder.
HostingRepository	PRIDE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_01:34:10.175.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilke Sen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-28 06:34:05	ID requested
⏵ 1	2025-04-22 01:34:10	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, lncRNA interactors, RNA-binding proteins

Anna Krook
contact affiliation	Professor, Department of Physiology and Pharmacology, Division of Integrative Physiology, Karolinska Institute, Stockholm, Sweden
contact email	anna.krook@ki.se
lab head
Ilke Sen
contact affiliation	Karolinska Institute, Stockholm Mondor Biomedical Reseach Institute, Paris
contact email	ilke.sen@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053490

PRIDE project URI

• PRIDE
  1. PXD053490
     1. Label: PRIDE project
     2. Name: Downregulation of Human-Specific lncRNA TMEM9B-AS1 in Skeletal Muscle of People with Type 2 Diabetes Impacts Ribosomal Biogenesis