PXD053480

PXD053480 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteins that interact with PHAX and UAP56 from HeLa nuclear extracts
Description	HeLa nuclear extracts and RNase A were incubated with GST-PHAX and FLAG-UAP56 in the presence of ATP. The mixture was subjected to immunoprecipitation. The bound proteins were eluted with FLAG peptide. The eluate was subjected to GST pull-down. The bound proteins were treated with HRV 3C protease. Supernatants were analysed by SDS-PAGE and mass spectrometry.
HostingRepository	jPOST
AnnounceDate	2025-06-28
AnnouncementXML	Submission_2025-06-27_08:00:05.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ichiro Taniguchi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-28 00:12:40	ID requested
⏵ 1	2025-06-27 08:00:06	announced

Publication List

Taniguchi I, Hirose T, Ohno M, The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA. Nucleic Acids Res, 52(17):10668-10682(2024) [pubmed]

Taniguchi I, Hirose T, Ohno M, The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA. Nucleic Acids Res, 52(17):10668-10682(2024) [pubmed]

Keyword List

submitter keyword: HeLa nuclear extract, GST pull-down, immunoprecipitation

submitter keyword: HeLa nuclear extract, GST pull-down, immunoprecipitation

Contact List

Ichiro Taniguchi
lab head
Ichiro Taniguchi
contact affiliation	Osaka University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003184/