PXD053470

PXD053470 is an original dataset announced via ProteomeXchange.

Title	Calcium-triggered (de)ubiquitination events in synapses
Description	Neuronal communication relies on neurotransmitter release from synaptic vesicles (SVs), whose dynamics are controlled by Ca2+-dependent pathways, as many thoroughly studied phosphorylation cascades. However, little is known about other post-translational modifications, as ubiquitination. To address this, we analysed resting and stimulated synaptosomes (isolated synapses) by quantitative mass spectrometry. We identified more than 5,000 ubiquitination sites on ~2,000 proteins, the majority of which participate in the SV recycling processes. Several proteins showed significant changes in ubiquitination in response to Ca2+ influx, with the most pronounced changes in CaMKIIα and the clathrin adaptor protein AP180. To validate this finding, we generated a CaMKIIα mutant lacking the ubiquitination target (K291), and analyse it in both neurons and non-neuronal cells. K291 ubiquitination influences CaMKIIα activity and synaptic function, by modulating its autophosphorylation at a functionally important site (T286). We suggest that activity-dependent ubiquitination is an important regulator of synaptic function.
HostingRepository	PanoramaPublic
AnnounceDate	2025-04-19
AnnouncementXML	Submission_2025-04-19_17:15:34.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sofia Ainatzi
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	GG; Phospho; Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-27 19:58:22	ID requested
⏵ 1	2025-04-19 17:15:35	announced

Ainatzi S, Kaufmann SV, Silbern I, Georgiev SV, Lorenz S, Rizzoli SO, Urlaub H, -Triggered (de)ubiquitination Events in Synapses. Mol Cell Proteomics, 24(4):100946(2025) [pubmed]

submitter keyword: synapse, ubiquitination, CaMKIIα, phosphorylation

Henning Urlaub
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	henning.urlaub@mpinat.mpg.de
lab head
Sofia Ainatzi
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	sainatzi5@gmail.com
dataset submitter

Panorama Public dataset URI