PXD053452

PXD053452 is an original dataset announced via ProteomeXchange.

Title	BioID of the proteins CGP and FRM1 from Toxoplasma gondii
Description	The conoid complex, a unique structure in the phylum Apicomplexa, is crucial for host cell invasion, egress, and motility. Despite its importance, the detailed molecular composition and assembly mechanisms of the conoid complex remain poorly understood. In previous research, we identified an essential conoidal protein, CGP, which is required for parasite motility activation through a splitCas9 phenotypic screen. Here, using proximity-dependent biotin identification of CGP and FRM1, we uncovered novel components localizing at the preconoidal rings. Furthermore, we identified a novel daughter-specific protein localizing at the APRs. Depletion of this protein resulted in severe morphological defects and failure in daughter bud formation. Ultrastructure expansion microscopy revealed highly disorganized or absent nascent tubulin. Although conoidal proteins could form, they were defective in assembling the conoid complex. Collectively, our findings identify a novel daughter-specific apical polar ring protein that provides the base for the initiation and growth of nascent tubulin, which in turn provides the scaffold for daughter bud formation.
HostingRepository	PRIDE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_03:43:32.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-27 00:58:07	ID requested
⏵ 1	2025-09-17 03:43:33	announced

Dataset with its publication pending

submitter keyword: interactor, conoid complex,Toxoplasma gondii, CGP, FRM1

Prof. Dr. rer. nat. Markus Meissne
contact affiliation	Department of Experimental Parasitology LMU München Lena-Christ-Str. 48 82152 Planegg-Martinsried Telefon: +49 (0) 89 2180 - 3622 Fax: +49 (0) 89 2180 - 3623 E-Mail: markus.meissner@lmu.de
contact email	Markus.Meissner@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD053452

PRIDE project URI

• PRIDE
  1. PXD053452
     1. Label: PRIDE project
     2. Name: BioID of the proteins CGP and FRM1 from Toxoplasma gondii