PXD053438

PXD053438 is an original dataset announced via ProteomeXchange.

Title	HeLa Pro-IL-1a TurboID LC-MS/MS
Description	Pro-IL-1a is a pro-inflammatory cytokine that is localised to the nucleus in humans due to the presence of a nuclear localisation sequence in its pro-domain. However, some mammals have a predicted loss of fucntion to their nuclear localistion sequence. It is suggested that pro-IL-1a serves as a dual action protein, and that in addition to its role as a cytokine, it has an additional nuclear role. Therefore, we used biotin proximity labelling to explore the protein interactome of pro-IL-1a with the aim to investogate possible additional roles of this protein. We have suggested in our study that interaction with histone acetyl trasnferases may suggest a possible nuclear function of pro-IL-1a.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:49.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rose Wellens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 13:20:30	ID requested
1	2024-06-28 06:33:30	announced
2	2024-08-19 05:00:43	announced	2024-08-19: Updated project metadata.
⏵ 3	2024-10-22 06:47:50	announced	2024-10-22: Updated project metadata.

10.1038/s41467-024-50901-0;

Wellens R, Tapia VS, Seoane PI, Bennett H, Adamson A, Coutts G, Rivers-Auty J, Lowe M, Green JP, Lopez-Castejon G, Brough D, Hoyle C, reveals evolutionary conserved nuclear interactions. Nat Commun, 15(1):6750(2024) [pubmed]

submitter keyword: inflammation, TurboID, biotin ligase proximity labelling,Pro-Interleukin-1 alpha, histone acetyl transferase, LC-MS/MS

David Brough
contact affiliation	Division of Neuroscience, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PT, UK. Geoffrey Jefferson Brain Research Centre, The Manchester Academic Health Science Centre, Northern Care Alliance NHS Group, University of Manchester, Manchester, M13 9PT, UK. The Lydia Becker Institute of Immunology and Inflammation, University of Manchester, Manchester, M13 9PT, UK.
contact email	david.brough@manchester.ac.uk
lab head
Rose Wellens
contact affiliation	University of Manchester
contact email	rose.a.wellens@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD053438

PRIDE project URI

• PRIDE
  1. PXD053438
     1. Label: PRIDE project
     2. Name: HeLa Pro-IL-1a TurboID LC-MS/MS