PXD053436

PXD053436 is an original dataset announced via ProteomeXchange.

Title	Breast cancer-derived extracellular vesicles modulate the cytoplasmic metabolism and cytoskeletal mechanics of blood-brain barrier endothelial cells
Description	Normal cerebral vasculature is characterized by high selectivity, low permeability and low transcytosis rates, ensuring proper brain physiology. Our group has demonstrated that extracellular vesicles isolated from brainseeking MDA-MB-231 cells (Br-EVs) breach the intact BBB in vivo and significantly increase the incidence of breast-to-brain metastasis, suggesting a dysfunction of the cerebral microvasculature. To elucidate the mechanisms by which Br-EVs modify the BBB and promote breast-to-brain metastasis, we utilized a quantitative global proteomic approach to assess changes in protein expression within murine cerebral microvessels post-Br-EV treatment. We employed label-free mass spectrometry-based quantitative proteomics to analyze the protein content of cerebral microvessels from the brains of mice treated with PBS, P-EVs or Br-EVs.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_20:35:39.072.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Busatto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 11:21:38	ID requested
⏵ 1	2025-05-06 20:35:39	announced

10.1002/jev2.70038;

Busatto S, Song TH, Kim HJ, Hallinan C, Lombardo MN, Stemmer-Rachamimov AO, Lee K, Moses MA, Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells. J Extracell Vesicles, 14(1):e70038(2025) [pubmed]

submitter keyword: breast cancer, exosomes, microvesicles,Extracellular vesicles, pre-metastatic niche, brain metastasis, blood-brain barrier

Marsha Moses
contact affiliation	1) Vascular Biology Program, Boston Children's Hospital, Boston, MA, United States 2) Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States
contact email	marsha.moses@childrens.harvard.edu
lab head
Sara Busatto
contact affiliation	Harvard Medical School Boston Children's Hospital
contact email	sara.busatto@childrens.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053436

PRIDE project URI

• PRIDE
  1. PXD053436
     1. Label: PRIDE project
     2. Name: Breast cancer-derived extracellular vesicles modulate the cytoplasmic metabolism and cytoskeletal mechanics of blood-brain barrier endothelial cells