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PXD053436

PXD053436 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBreast cancer-derived extracellular vesicles modulate the cytoplasmic metabolism and cytoskeletal mechanics of blood-brain barrier endothelial cells
DescriptionNormal cerebral vasculature is characterized by high selectivity, low permeability and low transcytosis rates, ensuring proper brain physiology. Our group has demonstrated that extracellular vesicles isolated from brainseeking MDA-MB-231 cells (Br-EVs) breach the intact BBB in vivo and significantly increase the incidence of breast-to-brain metastasis, suggesting a dysfunction of the cerebral microvasculature. To elucidate the mechanisms by which Br-EVs modify the BBB and promote breast-to-brain metastasis, we utilized a quantitative global proteomic approach to assess changes in protein expression within murine cerebral microvessels post-Br-EV treatment. We employed label-free mass spectrometry-based quantitative proteomics to analyze the protein content of cerebral microvessels from the brains of mice treated with PBS, P-EVs or Br-EVs.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_20:35:39.072.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Busatto
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-26 11:21:38ID requested
12025-05-06 20:35:39announced
Publication List
10.1002/jev2.70038;
Busatto S, Song TH, Kim HJ, Hallinan C, Lombardo MN, Stemmer-Rachamimov AO, Lee K, Moses MA, Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells. J Extracell Vesicles, 14(1):e70038(2025) [pubmed]
Keyword List
submitter keyword: breast cancer, exosomes, microvesicles,Extracellular vesicles, pre-metastatic niche, brain metastasis, blood-brain barrier
Contact List
Marsha Moses
contact affiliation1) Vascular Biology Program, Boston Children's Hospital, Boston, MA, United States 2) Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States
contact emailmarsha.moses@childrens.harvard.edu
lab head
Sara Busatto
contact affiliationHarvard Medical School Boston Children's Hospital
contact emailsara.busatto@childrens.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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Repository Record List
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