PXD053436 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Breast cancer-derived extracellular vesicles modulate the cytoplasmic metabolism and cytoskeletal mechanics of blood-brain barrier endothelial cells |
Description | Normal cerebral vasculature is characterized by high selectivity, low permeability and low transcytosis rates, ensuring proper brain physiology. Our group has demonstrated that extracellular vesicles isolated from brainseeking MDA-MB-231 cells (Br-EVs) breach the intact BBB in vivo and significantly increase the incidence of breast-to-brain metastasis, suggesting a dysfunction of the cerebral microvasculature. To elucidate the mechanisms by which Br-EVs modify the BBB and promote breast-to-brain metastasis, we utilized a quantitative global proteomic approach to assess changes in protein expression within murine cerebral microvessels post-Br-EV treatment. We employed label-free mass spectrometry-based quantitative proteomics to analyze the protein content of cerebral microvessels from the brains of mice treated with PBS, P-EVs or Br-EVs. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-06_20:35:39.072.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sara Busatto |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-26 11:21:38 | ID requested | |
⏵ 1 | 2025-05-06 20:35:39 | announced | |
Publication List
10.1002/jev2.70038; |
Busatto S, Song TH, Kim HJ, Hallinan C, Lombardo MN, Stemmer-Rachamimov AO, Lee K, Moses MA, Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells. J Extracell Vesicles, 14(1):e70038(2025) [pubmed] |
Keyword List
submitter keyword: breast cancer, exosomes, microvesicles,Extracellular vesicles, pre-metastatic niche, brain metastasis, blood-brain barrier |
Contact List
Marsha Moses |
contact affiliation | 1) Vascular Biology Program, Boston Children's Hospital, Boston, MA, United States 2) Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, United States |
contact email | marsha.moses@childrens.harvard.edu |
lab head | |
Sara Busatto |
contact affiliation | Harvard Medical School
Boston Children's Hospital |
contact email | sara.busatto@childrens.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053436 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053436
- Label: PRIDE project
- Name: Breast cancer-derived extracellular vesicles modulate the cytoplasmic metabolism and cytoskeletal mechanics of blood-brain barrier endothelial cells