PXD053434

PXD053434 is an original dataset announced via ProteomeXchange.

Title	Elucidating molecular mechanisms underlying brain metastasis-promoting function of Cdk5
Description	To elucidate molecular mechanisms underlying brain metastasis-promoting function of Cdk5, two analyses were performed. In the first analysis, we screened for Cdk5 substrates in brain-seeking breast cancer cell lines 4T1.Br3 and T11.Br1, transfected with shRNA targeting Cdk5 mRNA (100% knockdown) or control. T11.Br1 and 4T1.Br3 cells (shCDK5 or shCtl) were cultured for 6 days in the presence of labelled arginine and lysine from the SILAC Protein Quantitation Kit. For the second analysis, we performed a quantitative label-free mass-spectrometry analysis of astrocytes’ conditioned media pre-educated by 4T1.Br3 cell-derived CM for 72 h. For the second analysis, we performed a quantitative label-free mass-spectrometry analysis of astrocytes’ conditioned media pre-educated by 4T1.Br3 cell-derived CM for 72 h.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:52.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antrix Jain
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 10:01:20	ID requested
1	2024-06-28 13:04:04	announced
⏵ 2	2024-10-22 06:47:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: astrocytes, Cdk5, phosphorylation,brain metastasis, kinase, T11, conditioned media, 4T1

Arseniy Yuzhalin
contact affiliation	MD Anderson Cancer Center, Houston, TX
contact email	ayuzhalin@mdanderson.org
lab head
Antrix Jain
contact affiliation	Baylor College of Medicine
contact email	antrixj@bcm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD053434

PRIDE project URI

• PRIDE
  1. PXD053434
     1. Label: PRIDE project
     2. Name: Elucidating molecular mechanisms underlying brain metastasis-promoting function of Cdk5