PXD053432

PXD053432 is an original dataset announced via ProteomeXchange.

Title	Identification of the proteins that stably or transiently interact with the nuclear poly(A)-binding protein Pab2 in the fission yeast Schizosaccharomyces japonicus using the proximity-labeling method TurboID.
Description	We looked for the proteins that associate with Pab2, a nuclear poly(A)-binding protein, in Schizosaccharomyces japonicus through the proximity-labeling technology TurboID followed by mass spectrometry. We revealed that Pab2 interacts with two putative RNA-binding proteins, Red5 and Rmn1, and a yeast two-hybrid assay verified these interactions. We also found that Pab2 is associated with the CPSF (mRNA cleavage and polyadenylation specificity factor) component Ppn1, and the Pab2-Ppn1 interaction was confirmed by a yeast two-hybrid assay (and Ppn1 TurboID). These results indicate that Pab2 works with the three proteins mentioned above in Schizosacccharomyces japonicus.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-12_23:06:09.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	子月 刘
SpeciesList	scientific name: Schizosaccharomyces; NCBI TaxID: NCBITaxon:4895; scientific name: Schizosaccharomyces japonicus; NCBI TaxID: 4897;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 07:35:12	ID requested
⏵ 1	2025-03-12 23:06:10	announced

Dataset with its publication pending

submitter keyword: Fission yeast,Nuclear poly(A)-binding protein, Schizosaccharomyces japonicus

Tomoyasu Sugiyama
contact affiliation	ShanghaiTech University - Zhangjiang Campus: ShanghaiTech University Shanghai, CHINA
contact email	tsugiyama@shanghaitech.edu.cn
lab head
子月 刘
contact affiliation	ShanghaiTech University
contact email	liuzy8@shanghaitech.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053432
     1. Label: PRIDE project
     2. Name: Identification of the proteins that stably or transiently interact with the nuclear poly(A)-binding protein Pab2 in the fission yeast Schizosaccharomyces japonicus using the proximity-labeling method TurboID.