PXD053426

PXD053426 is an original dataset announced via ProteomeXchange.

Title	Dbf4-Dependent Kinase Finetunes INO80 Function at Chromosome Replication Origins
Description	The highly conserved Dbf4-Dependent Kinase (DDK) plays a pivotal role in the nucleus during S phase, where it directly phosphorylates the replicative helicase, the minichromosome maintenance (MCM) complex. This leads to the initiation of chromosome replication. However, aside from the MCM complex, few other targets have been identified to date, leaving DDK an understudied kinase. Here, we describe a two-pronged mass spectrometry-based approach and define the nuclear DDK-dependent phosphoproteome, which consists of approximately 400 phosphorylation events. Within this network, we found that DDK directly phosphorylates the Arp8 subunit of the multi-subunit chromatin remodeler complex INO80. Arp8 phosphorylation stabilises INO80’s intramolecular complex integrity, which finetunes its nucleosome spacing activity at replication origins. This adjustment of origin chromatin architecture stimulates replication and is important for the response to replication stress. Our results represent a significant advance in our understanding of the molecular mechanisms underlying the regulation of replication origins.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_18:10:16.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shibojyoti Lahiri
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 07:04:19	ID requested
⏵ 1	2026-04-05 18:10:17	announced

Bansal P, Lahiri S, Kumar CN, Furtmeier J, Spechtenhauser L, Galanti L, Barba Tena JD, Chacin E, Linder G, Ort, í, z-Baz, á, n M Á, M, ü, ller M, Vizjak P, Straub T, Mueller-Planitz F, Stigler J, Aguilera A, G, ó, mez-Gonz, á, lez B, Pfander B, Korber P, Imhof A, Kurat CF, Dbf4-dependent kinase finetunes Ino80 function at chromosome replication origins. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-70698-4;

submitter keyword: Chromosome replication,Phosphoproteomics, INO80, Cross-link MS, Yeast DDK

Axel Imhof
contact affiliation	Histone Modifications Group Zentrallabor für Proteinanalytik BioMedical Center Faculty of Medicine Ludwig-Maximilians-University of Munich Germany
contact email	imhof@lmu.de
lab head
Shibojyoti Lahiri
contact affiliation	Ludwig-Maximilians-University of Munich
contact email	shibojyoti.lahiri@med.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053426
     1. Label: PRIDE project
     2. Name: Dbf4-Dependent Kinase Finetunes INO80 Function at Chromosome Replication Origins