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PXD053413

PXD053413 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control the plant pest nematodes Meloidogyne javanica and M. incognita
DescriptionThe global imperative to enhance crop protection while preserving the environment has increased interest in the application of biological pesticides. Bacillus thuringiensis (Bt) is a Gramm-positive bacterium that can produce nematicidal proteins and accumulate them in parasporal crystals. Root-knot nematodes are obligate root plant parasitic which are distributed worldwide, causing severe damages to the infested plants and, consequently, large yield reductions. In this work, we have evaluated the toxicity of the crystal proteins Cry5, Cry21, App6, and Xpp55 against two root-knot nematodes belonging to the Meloidogyne genus (M. incognita and M. javanica). The results show that all four proteins, when solubilized, were highly toxic for both nematode species. To check the potential of using Bt strains producing nematicidal crystal proteins as biopesticides to control plant parasitic nematodes in the field, in planta assays were conducted, using two wild Bt strains which produced Cry5 or a combination of App6 and Cry5 proteins. The tests were carried out with cucumber or with tomato plants infested with M. javanica J2, subjected to irrigation with spore+cristal mixtures of the respective strains. The results showed that the efficacy of the nematicidal activity was plant-dependent, as Bt was able to reduce emerged J2 in tomato plants but not in cucumber plants. In addition, the toxicity observed in the in planta assays was much lower than expected, highlighting the challenge of the crystal proteins to exert their toxicity. This emphasizes the delivery of the Bt proteins as crucial for its use to control root-knot nematodes.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_20:31:25.725.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuz Valero
SpeciesList scientific name: Bacillus thuringiensis; NCBI TaxID: 1428;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-26 03:12:55ID requested
12025-05-06 20:31:26announced
Publication List
Bel Y, Galeano M, Ba, ñ, os-Salmeron M, Andr, é, s-Ant, ó, n M, Escriche B, Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control Meloidogyne javanica and M. incognita. Appl Microbiol Biotechnol, 108(1):525(2024) [pubmed]
10.1007/s00253-024-13365-2;
Keyword List
submitter keyword: Bio-control, bioassay, in vitro assay, Bt wild strains, in planta assay
Contact List
Baltasar Escriche
contact affiliationInstitute BIOTECMED / Department of Genetics, University of Valencia, Burjassot, Spain
contact emailbaltasar.escriche@uv.es
lab head
Luz Valero
contact affiliationUniversitat de Valencia
contact emailmluz.valero@uv.es
dataset submitter
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Dataset FTP location
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