PXD053413

PXD053413 is an original dataset announced via ProteomeXchange.

Title	Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control the plant pest nematodes Meloidogyne javanica and M. incognita
Description	The global imperative to enhance crop protection while preserving the environment has increased interest in the application of biological pesticides. Bacillus thuringiensis (Bt) is a Gramm-positive bacterium that can produce nematicidal proteins and accumulate them in parasporal crystals. Root-knot nematodes are obligate root plant parasitic which are distributed worldwide, causing severe damages to the infested plants and, consequently, large yield reductions. In this work, we have evaluated the toxicity of the crystal proteins Cry5, Cry21, App6, and Xpp55 against two root-knot nematodes belonging to the Meloidogyne genus (M. incognita and M. javanica). The results show that all four proteins, when solubilized, were highly toxic for both nematode species. To check the potential of using Bt strains producing nematicidal crystal proteins as biopesticides to control plant parasitic nematodes in the field, in planta assays were conducted, using two wild Bt strains which produced Cry5 or a combination of App6 and Cry5 proteins. The tests were carried out with cucumber or with tomato plants infested with M. javanica J2, subjected to irrigation with spore+cristal mixtures of the respective strains. The results showed that the efficacy of the nematicidal activity was plant-dependent, as Bt was able to reduce emerged J2 in tomato plants but not in cucumber plants. In addition, the toxicity observed in the in planta assays was much lower than expected, highlighting the challenge of the crystal proteins to exert their toxicity. This emphasizes the delivery of the Bt proteins as crucial for its use to control root-knot nematodes.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_20:31:25.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Bacillus thuringiensis; NCBI TaxID: 1428;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 03:12:55	ID requested
⏵ 1	2025-05-06 20:31:26	announced

Bel Y, Galeano M, Ba, ñ, os-Salmeron M, Andr, é, s-Ant, ó, n M, Escriche B, Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control Meloidogyne javanica and M. incognita. Appl Microbiol Biotechnol, 108(1):525(2024) [pubmed]

10.1007/s00253-024-13365-2;

submitter keyword: Bio-control, bioassay, in vitro assay, Bt wild strains, in planta assay

Baltasar Escriche
contact affiliation	Institute BIOTECMED / Department of Genetics, University of Valencia, Burjassot, Spain
contact email	baltasar.escriche@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053413

PRIDE project URI

• PRIDE
  1. PXD053413
     1. Label: PRIDE project
     2. Name: Bacillus thuringiensis Cry5, Cry21, App6 and Xpp55 proteins to control the plant pest nematodes Meloidogyne javanica and M. incognita