PXD053406

PXD053406 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of sap reveals root-shoot communication dynamics in potato and barley following flg22 treatment
Description	Potato (Solanum tuberosum, variety Princ) and barley (Hordeum vulgare, variety Sebastian) plants were cultivated in a controlled environment. Plants were grown in Potgrond H soil (Klasmann-Deilmann GmbH, Germany) under a 12-hour photoperiod with a constant temperature of 21 °C and a photon flux density of 100 μmol m⁻² s⁻¹. Potato tubers were used as starting material, while barley plants were grown from surface-sterilized seeds. After six weeks of cultivation for potato and three weeks for barley, the sap proteome was collected by cutting the stems 10–20 mm above the soil surface. Root exudates were sampled into three fractions over a two-hour period: F1 (15 min), F2 (75 min), and F3 (135 min). Each experiment included at least three independent biological replicates. A subset of plants was pre-incubated with flg22, a conserved peptide sequence derived from bacterial flagellin (QRLSTGSRINSAKDDAAGLQIA, >95% purity; ProteoGenix, France). Flg22 solution (1 μM flg22, 0.025% v/v Silwet L-77) was applied by spraying the leaves and pouring the solution under the pot. Potato plants received 30 ml and 200 ml of the solution for spraying and watering, respectively. Due to their smaller size, barley plants were treated with half the solution volume. Mock-treated plants received a solution containing only 0.025% Silwet L-77. After 24 hours, plants were cut, and the third fraction of the sap (75-135 min) was collected. Root and shoot tissues were collected in liquid nitrogen, the sap was collected as described above. Each experiment included at least three independent biological replicates.
HostingRepository	PRIDE
AnnounceDate	2024-07-20
AnnouncementXML	Submission_2024-07-20_05:34:23.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-25 15:18:32	ID requested
⏵ 1	2024-07-20 05:34:23	announced

10.3390/PLANTS13141983;

submitter keyword: extracellular proteins, exudates,xylem sap, flg22

Martin Cerny
contact affiliation	Mendel Universitz in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD053406

PRIDE project URI

• PRIDE
  1. PXD053406
     1. Label: PRIDE project
     2. Name: Proteomic analysis of sap reveals root-shoot communication dynamics in potato and barley following flg22 treatment