PXD053401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct intersecting pathways link homolog pairing to initiation of meiotic chromosome synapsis |
Description | Faithful meiotic segregation requires pairwise alignment of the homologous chromosomes and Synaptonemal Complex assembly (SC) at their interface. Here, we investigate on new factors that promote and coordinate these events during C. elegans meiosis. We identify BRA-2 (BMP Receptor Associated family member 2) as an interactor of HIM-17, previously shown to promote double-strand break formation. We found that loss of bra-2 specifically impairs synapsis licensing without affecting homologs recognition, SC maintenance or chromosome movement. Double mutant analysis revealed a previously unrecognized role for HIM-17 in promoting homolog pairing under dysfunctional SC assembly, without perturbing nuclear envelope recruitment of factors required for chromosome movement. We provide evidence that bra-2 and him-17 act in distinct pathways, exerting partially redundant functions in SC licensing, as well as separable roles in regulating homologs pairing. Altogether, our findings unveil novel mechanisms that ensure stabilization of homologous chromosome interaction via SC licensing upon homology assessment. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-14 |
AnnouncementXML | Submission_2025-03-14_02:34:30.695.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katerina Hanakova |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-25 09:27:41 | ID requested | |
⏵ 1 | 2025-03-14 02:34:31 | announced | |
Publication List
Keyword List
submitter keyword: Caenorhabditis elegans, BRA-2, HIM-17, LC-MS/MS |
Contact List
Zbynek Zdrahal |
contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
Katerina Hanakova |
contact affiliation | CEITEC MU |
contact email | katerina.hanak@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053401
- Label: PRIDE project
- Name: Distinct intersecting pathways link homolog pairing to initiation of meiotic chromosome synapsis