PXD053394

PXD053394 is an original dataset announced via ProteomeXchange.

Title	Maintaining Basal B-RAF Kinase Activity and ABA signaling via Single Serine Dephosphorylation and Autophosphorylation
Description	The phytohormone abscisic acid (ABA) regulates plant responses to environmental stresses, development, and immunity. Under unfavorable conditions, ABA forms a complex with its receptor proteins PYR1/PYLs/RCARs, inhibiting A clade PP2C phosphatases and releasing SnRK2s from PP2C-mediated inhibition. RAF kinases from the B1, B2, and B3 subgroups phosphorylate and reactivate SnRK2s, initiating ABA responses. While ABA does not significantly activate B-RAFs, their basal activity is essential for initiating ABA signaling. However, the mechanisms sustaining this basal B-RAF activity are not fully understood. In this study, we revealed that A clade PP2Cs interact with and dephosphorylate B3 subgroup RAFs at a conserved serine residue, corresponding to Ser619 in RAF3, within the phosphate-binding loop. A phosphomimicking mutation at this residue, RAF3S619D, failed to bind ATP and exhibited diminished kinase activity in vitro and in vivo.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:15:01.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chen zhu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	biotinylated residue; phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-25 05:23:54	ID requested
⏵ 1	2025-11-09 16:15:02	announced

10.1111/jipb.70012;

Zhu C, Sang T, Zhang Z, Wang Y, Lin Z, Wang W, Lang Z, Zhu JK, Wang P, Maintaining basal B-RAF kinase activity for abscisic acid signaling via reciprocal phosphoregulation of a single serine residue. J Integr Plant Biol, 67(11):2848-2862(2025) [pubmed]

submitter keyword: Arabidopsis, Protein phosphatase 2C(PP2C), abiotic stress,Abscisic acid (ABA), Raf kinase, Protein phosphorylation

Pengcheng Wang
contact affiliation	Institute of Advanced Biotechnology and School of Medicine, Southern University of Science and Technology
contact email	wangpc@sustech.edu.cn
lab head
chen zhu
contact affiliation	Shanghai Center for Plant Stress Biology
contact email	chenzhu@psc.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053394
     1. Label: PRIDE project
     2. Name: Maintaining Basal B-RAF Kinase Activity and ABA signaling via Single Serine Dephosphorylation and Autophosphorylation