PXD053392

PXD053392 is an original dataset announced via ProteomeXchange.

Title	Identification of Scedosporium boydii specific antigens with potential diagnostic interest in patients with cystic fibrosis
Description	Fungal infections pose a significant threat to patients with cystic fibrosis (pwCF). Particularly those caused by Scedosporium/Lomentospora species, being the second most common filamentous fungi in the respiratory tract of these patients after Aspergillus. The current serodiagnostic methods for detecting Scedosporium/Lomentospora, such as Counterimmunoelectrophoresis and ELISA using crude extracts, are neither standardized nor commercially available, and they suffer from cross-reactivity with other fungal species. This study aimed to identify specific antigens of Scedosporium boydii with potential utility for serodiagnosis in pwCF. The antigenic profile of S. boydii was analyzed by combining Two-Dimensional Polyacrylamide Gel Electrophoresis and immunoblotting with sera from pwCF with microbiological cultures of respiratory samples positive for Scedosporium/Lomentospora (Scedo+), and sera from mice intravenously infected with S. boydii. The fungal antigens specifically recognized by pwCF Scedo+ were: 6 phosphogluconate dehydrogenase (Pgd), 40S ribosomal protein S1 (Rps1) and S18 (Rps18), Small glutamine-rich tetratricopeptide repeat-containing protein (Sgta), Cytochrome c peroxidase (Ccp), Mannitol-1-phosphate 5-dehydrogenase (Mpd), L xylulose reductase (Lxr), Tetrahydroxynaphthalene reductase (Thnr), Heat shock 70 kDa protein (Hsp70), Proteasome subunits alpha (Psma6) and beta (Psmb2), Peroxiredoxin-1 (Prdx1), ATP dependent Clp protease proteolytic subunit (Clpp), 3 ketoacyl-CoA thiolase (Kat), 4 aminobutyrate aminotransferase (Abat), Succinyl CoA:3-ketoacid CoA transferase 1 (Scot) and Phosphoenolpyruvate carboxykinase (Pck). The fungal antigens specifically recognized by infected mice were: Hsp70, S5 DRBM domain-containing ribosomal protein (Rps5), mitochondrial NFU1 iron-sulfur cluster scaffold-like protein (Nfu1), 60S ribosomal protein L8 (Rpsl8), phosphomannomutase (Pmm) and proteasome subunit alpha type 5 protein (Psma5).
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:21:16.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aitziber Antoran
SpeciesList	scientific name: Fungi; NCBI TaxID: NCBITaxon:4751; scientific name: Scedosporium boydii; NCBI TaxID: 5597;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-25 04:32:26	ID requested
⏵ 1	2025-09-28 16:21:17	announced

10.1021/acs.jproteome.5c00260;

Martin-Souto L, Abio-Dorronsoro L, Areitio M, Aparicio-Fernandez L, Rodriguez-Erenaga O, Martin-Gomez MT, Antoran A, Rementeria A, Buldain I, Ramirez-Garcia A, Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients. J Proteome Res, ():(2025) [pubmed]

submitter keyword: Fungi, Serodiagnosis, Antigens, Scedosporium/Lomentospora, Cystic Fibrosis

Andoni Ramirez
contact affiliation	Department of Immunology, Microbiology and Parsitology Faculty of Science and Technology University of the Basque Country (UPV/EHU)
contact email	andoni.ramirez@ehu.eus
lab head
Aitziber Antoran
contact affiliation	University of the Basque Country (UPV/EHU)
contact email	aitziber.antoran@ehu.eus
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053392
     1. Label: PRIDE project
     2. Name: Identification of Scedosporium boydii specific antigens with potential diagnostic interest in patients with cystic fibrosis