PXD053391

PXD053391 is an original dataset announced via ProteomeXchange.

Title	A classic famous prescription alleviates inflammation in CUMS model mice via modulating MYDGF/MAP4K4/NF-κB signaling pathway, verified through UPLC-HRMS and proteomics analysis
Description	Major depressive disorder (MDD) has caused a heavy burden on society and affected over 350 million patients worldwide currently. However, there have been 30-50% MDD patients with non-response to antidepressants. Chronic unpredictable mild stress (CUMS) has been considered as a critical trigger factor for MDD, the pathological mechanism of which is strongly associated with stimulating inflammatory responses. Xiaoyaosan (XYS), as a classic famous prescription, has been proven effective for alleviating CUMS induced injury. Purpose: This study aimed to clarify the potential anti-inflammatory mechanisms of XYS treating CUMS model mice. Study design and methods: The therapeutic effects of XYS on the behavior and inflammatory indicators of CUMS model mice were determined. Ultra high performance liquid chromatography - High resolution mass spectrometry (UPLC-HRMS) was applied identify the compounds of XYS and proteomics analysis was utilized to explore the potential mechanism. Additionally, molecular docking and further in vivo experiments were conducted to verify the results of proteomics analysis. Results: The in vivo experiments confirmed XYS not only could significantly improve sucrose preference percentage and behavioral indicators of open field test, but also could decrease the serum levels of inflammatory indexes (CORT, hs-CRP, NFκB, FIB and Lp-PLA2) and inhibit the protein expressions of IL-6 and TNF-α in brain of CUMS model mice. Proteomics analysis presented that MAPK signaling pathway might be the important anti-inflammatory mechanism of XYS alleviating CUMS induced injury. UPLC-HRMS identify the compounds of XYS, which were taken into molecular docking to analysis the interaction between XYS and MYDGF, PKC, MAP4K4, P-p65, p65, P-IKBα, and IKBα. It was validated that XYS could exert regulatory effects on the MYDGF/MAP4K4/NF-κB signaling pathway in CUMS model mice. Conclusion: In conclusion, this study first demonstrated that XYS could alleviate inflammation in CUMS model mice via modulating MYDGF/MAP4K4/NF-κB signaling pathway.
HostingRepository	iProX
AnnounceDate	2024-06-25
AnnouncementXML	Submission_2024-06-25_03:08:51.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruolan Huang
SpeciesList	scientific name: Mus sp.; NCBI TaxID: 10095;
ModificationList	No PTMs are included in the dataset
Instrument	Progenesis LC-MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-25 03:07:37	ID requested
⏵ 1	2024-06-25 03:08:52	announced

Dataset with its publication pending

submitter keyword: Herbal medicine, Chronic unpredictable mild stress, Major depressive disorder, Proteomics analysis, Ultra high performance liquid chromatography - High resolution mass spectrometry

Xifei Yang
contact affiliation	Shenzhen Key Laboratory of Modern Toxicology
contact email	xifeiyang@gmail.com
lab head
Ruolan Huang
contact affiliation	Shenzhen University General Hospital
contact email	ruolan_huang@szu.edu.cn
dataset submitter

iProX dataset URI