⮝ Full datasets listing
PXD053368
PXD053368 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-translational profiling in the cardiac endothelium in response to LPS-induced inflammation in female mice in vivo: a proof-of-concept approach |
| Description | Producing functional proteins involves multiple steps during mRNA translation on the ribosomes. However, co-translational regulatory mechanisms remain poorly characterized in intact mammalian systems. As a proof-of-concept, we developed a multi-omics approach to investigate endothelial-specific, co-translational regulation by modifying the translating ribosome affinity purification (TRAP) in vivo. We simultaneously co-immunoprecipitated (IP) polysome-associated mRNAs and proteins from the hearts of hemagglutinin-tagged ribosomal protein L22 mice (RiboTag) crossed with inducible endothelial-specific Cdh5CreERT2 mice (RiboTagEC). To perturb endothelial function, female mice were injected with E. coli lipopolysaccharide (LPS) (6 mg/Kg, i.p., 12 h). Hearts were homogenized, with ~?10% used for input RNA-Seq and proteomics controls, and the remainder for IP of ribosome-bound polyadenylated mRNA and proteins. Endothelial cell transcripts (pecam1, cdh5) were enriched?>?5-fold, while markers characteristic of other cell types were significantly depleted (?1250 overlapping terms) to identify pathways associated with concordant and discordant co-translational regulation. LPS was identified as the upstream regulator of the co-translational dataset that was concordantly regulated. Upregulated mRNAs but not proteins related to glycolysis were discordantly regulated. These findings validate our proof-of-concept multi-omics approach as a predictive platform for identifying disease-relevant pathways regulated at the co-translational level in vivo. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-19 |
| AnnouncementXML | Submission_2026-06-19_04:46:19.184.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Chad M. Warren |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Carbamyl; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-24 11:47:11 | ID requested | |
| ⏵ 1 | 2026-06-19 04:46:20 | announced |
Publication List
| Warren CM, Swaminathan B, Langa P, Villa SR, Thompson WC, Chrzanowska M, Kitajewski JK, Solaro RJ, Wolska BM, Goldspink PH, Co-translational profiling in the cardiac endothelium in response to LPS-induced inflammation in female mice in vivo: a proof-of-concept approach. Mol Cell Biochem, 481(6):2381-2398(2026) [pubmed] |
Keyword List
| submitter keyword: heart, translatome, Endothelium, Lipopolysaccharide, Ribosome |
Contact List
| Paul H. Goldspink | |
|---|---|
| contact affiliation | University of Illinois Chicago |
| contact email | pgolds@uic.edu |
| lab head | |
| Chad M. Warren | |
| contact affiliation | University of Illinois-Chicago |
| contact email | cmwarren@uic.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
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