PXD053351

PXD053351 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	RNAPII interactome in INTS6 degron cells
Description	In this study we show that acute INTS6 depletion remodels the RNAPII interactome and enhances ARMC5 binding, the same as what we observed in Ints6 KO cells. To investigate how acute INTS6 depletion affects RNAPII function, we determined the RNAPII interactome in the the Ints6 degron cells. Chromatin fractions from DMSO and dTAG treated Ints6 degron cells (treated for 1 hour) were analyzed by RNAPII IP-mass spectrometry. There are 12 samples in this MS run, samples 6-dTAG repeats1-3 and 6-DMSO repeats 1-3 are involved in the paper.
HostingRepository	PRIDE
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-21_16:32:27.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-24 04:42:12	ID requested
⏵ 1	2025-12-21 16:32:28	announced

Publication List

10.1016/j.molcel.2024.10.012;
Blears D, Lou J, Fong N, Mitter R, Sheridan RM, He D, Dirac-Svejstrup AB, Bentley D, Svejstrup JQ, Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint. Mol Cell, 84(24):4790-4807.e11(2024) [pubmed]

10.1016/j.molcel.2024.10.012;

Blears D, Lou J, Fong N, Mitter R, Sheridan RM, He D, Dirac-Svejstrup AB, Bentley D, Svejstrup JQ, Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpoint. Mol Cell, 84(24):4790-4807.e11(2024) [pubmed]

Keyword List

submitter keyword: transcription, INTS6,chromatin, RNA-polymerase II

submitter keyword: transcription, INTS6,chromatin, RNA-polymerase II

Contact List

Jesper Svejstrup
contact affiliation	DNRF Center for Gene Expression Department of Cellular and Molecular Medicine Blegdamsvej 3B University of Copenhagen 2200 Copenhagen N, Denmark
contact email	jsvejstrup@sund.ku.dk
lab head
Michael Wierer
contact affiliation	Proteomics Research Infrastructure, University of Copenhagen
contact email	michael.wierer@sund.ku.dk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD053351

PRIDE project URI

Repository Record List

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