PXD053343

PXD053343 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of proteins in SDS-PAGE gel by LC-MSMS
Description	Recombinant antibodies were purified from culture supernatants derived from the Dictyostelium discoideum model and loaded into an SDS-PAGE electrophoresis gel. The electrophoretic profile of the anti-alpha tubulin, anti-CISD1, and anti-CD8β antibodies reveals a band at 35 kDa, likely corresponding to a degradation product of the recombinant antibody. Additionally, a non-specific band appears under all conditions at 170 kDa. The objective of this analysis was to characterize these bands by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-11_07:19:27.381.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cyril Guilhen
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-24 01:43:24	ID requested
⏵ 1	2025-04-11 07:19:27	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Dictyostelium discoideum, recombinant antibodies

submitter keyword: Dictyostelium discoideum, recombinant antibodies

Contact List

Cyril Guilhen
contact affiliation	Biomedical Sciences Faculty of Medicine University of Geneva
contact email	Cyril.guilhen@unige.ch
lab head
Cyril Guilhen
contact affiliation	University of Geneva, Faculty of Medicine, Dpt of Cell Physiology and Metabolism, Centre Médical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
contact email	Cyril.guilhen@unige.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053343
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053343

PRIDE project URI

Repository Record List

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