PXD053341

PXD053341 is an original dataset announced via ProteomeXchange.

Title	photo-crosslinking mass spectrometry localises SNAPC5 and SNAPC2 in SNAPc
Description	RNA polymerase III (Pol III) is specialized in the transcription of short, functional RNAs, including small nuclear RNAs (snRNAs). At snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc)forming apreinitiation complex (PIC). Uniquely, SNAPc forms PICs with both Pol II and Pol IIIat their respectivepromoters. Increasingly, snRNA PIC structures arethe focus of structural characterization, however the mechanism of SNAPc cross-polymerase engagementand the role of the SNAPC2 and SNAPC5 subunits in transcription remain unclear. Here, we present CryoEM structuresoftheSNAPc-containing Pol III PIC assembled on the U6 snRNA promoterin the open and melting statesat 3.2-4.2Å resolution. Comparative structural analysis revealedunexpected differences with the corresponding yeast complex and revealedthe basis of SNAPc engagement with both Pol III and Pol IIPICs. Integrating crosslinkingmass spectrometry,we alsolocalizethe SNAPC2 and SNAPC5 subunitsin proximity to the bound promoter DNA, expanding upon existing descriptions of snRNA Pol III PIC structure.
HostingRepository	PRIDE
AnnounceDate	2025-01-06
AnnouncementXML	Submission_2025-01-06_08:17:09.428.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053341
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Graziadei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-24 00:36:13	ID requested
⏵ 1	2025-01-06 08:17:09	announced

10.6019/PXD053341;

10.1038/S41467-024-55553-8;

submitter keyword: POLIII, crosslinking MS, XL-MS, RNA polymerase III, CX-MS,SNAPC, SDA

Andrea Graziadei
contact affiliation	Human Technopole
contact email	andrea.graziadei@fht.org
lab head
Andrea Graziadei
contact affiliation	Human Technopole
contact email	andrea.graziadei@fht.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053341
     1. Label: PRIDE project
     2. Name: photo-crosslinking mass spectrometry localises SNAPC5 and SNAPC2 in SNAPc