PXD053328

PXD053328 is an original dataset announced via ProteomeXchange.

Title	Evaluation And Characterization Of Acute Respiratory Distress Syndrome In Tree Shrews Through TMT Proteomic Method
Description	Acute respiratory distress syndrome (ARDS), a common cause of acute fatal respiratory, is characterized by severe inflammatory lung injury as well as hallmarks of increased pulmonary vascular permeability, neutrophil infiltration, and macrophage accumulation. Tree shrew, a squirrel-like small animal model, has been confirmed more similar traits to human ARDS with one-hit intratracheal instillation of LPS in our previous study. In this study, we characterized protein profile changes induced by intranasal LPS challenge in the tree shrew model through tandem mass tag (TMT)-based quantitative proteomics and type II alveolar epithelial cells through pathological analysis. In total, 4070 proteins (p < 0.05) were identified from lung tissues of the LPS-induced group and PBS group. Among the differential expression proteins (DEPs) detected by t-test (≥|1.5-fold|), 529 DEPs were identified, of which 304 were upregulated, and 225 were downregulated. The most important pathways involved in the process of ARDS had been identified by enrichment analysis: oxidative stress, apoptosis, inflammatory responses, and vascular endothelial injury. In addition, proteins have been reported in animal models or clinical patients also detail investigated for further analysis, such as ceruloplasmin (CP), hemopexin (HPX), sphingosine kinase 1 (SphK1), lactotransferrin (LTF), and myeloperoxidase (MPO) were upregulated in induced tissues and confirmed by western blot analysis. Overall, this study not only reveals a comprehensive proteomic analysis of the ARDS tree shrew model but also provides novel insights into multi-pathways responses induced by the LPS challenge of tree shrews. We highlight shared and unique proteomic changes in the lungs of ARDS tree shrews and identify novel pathways for acute lung injury, which may promote the model into basic research and translational research.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_20:01:54.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenbo Cheng
SpeciesList	scientific name: Tupaia chinensis; NCBI TaxID: 246437;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-23 22:44:53	ID requested
⏵ 1	2025-05-06 20:01:55	announced

Xiong J, Zhang L, Xing J, Huang W, Wang N, Lin X, He S, Liao M, He J, Evaluation and Characterization of Acute respiratory distress syndrome in tree shrews through TMT proteomic method. PLoS One, 20(4):e0319752(2025) [pubmed]

10.1371/journal.pone.0319752;

submitter keyword: acute respiratory distress syndrome

tree shrew

TMT proteomics

lungs

immunological responses

Jun He
contact affiliation	Jinan University
contact email	hejun@jnu.edu.cn
lab head
Wenbo Cheng
contact affiliation	Shanghai Omicsspace Bioteh CO., LTD
contact email	wbcheng@omicsspace.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053328
     1. Label: PRIDE project
     2. Name: Evaluation And Characterization Of Acute Respiratory Distress Syndrome In Tree Shrews Through TMT Proteomic Method