PXD053315

PXD053315 is an original dataset announced via ProteomeXchange.

Title	A high-throughput screening approach identified a selective pro-caspase-10 probe that can inhibit its activity
Description	Caspases are cysteine proteases that play a critical role in inflammation and apoptosis. Caspases have a functional role in either the intrinsic apoptotic pathway as initiators of apoptosis or in the extrinsic apoptotic pathway as executioners of apoptosis. Caspase-10 has been speculated to play a principal role as an initiator of apoptosis but the absence of adequate tools, i.e. selective probes, have hindered our understanding of this enzyme as well as the other caspases. Here, we engineered a tobacco etch virus (TEV)-cleavable construct that is compatible with high-throughput screening to identify selective caspase-10 inhibitors. We identified a set of promiscuous caspase inhibitors, including pifithrin-µ (PFT), an inhibitor of p53 transcriptional activity, as well as promising selective pro-caspase-10 and TEV protease inhibitors.
HostingRepository	PRIDE
AnnounceDate	2025-02-04
AnnouncementXML	Submission_2025-02-03_19:53:10.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jose Castellon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-23 15:32:18	ID requested
⏵ 1	2025-02-03 19:53:11	announced

Dataset with its publication pending

submitter keyword: caspase-10,HTS, biotin azide

Dr. Keriann M. Backus
contact affiliation	Assistant Professor, Chemistry and Biochemistry, Biological Chemistry Member, Biochemistry, Biophysics & Structural Biology GPB Home Area (lab head)
contact email	KBackus@mednet.ucla.edu
lab head
Jose Castellon
contact affiliation	UCLA
contact email	joseomar805@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053315

PRIDE project URI

• PRIDE
  1. PXD053315
     1. Label: PRIDE project
     2. Name: A high-throughput screening approach identified a selective pro-caspase-10 probe that can inhibit its activity