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PXD053315

PXD053315 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA high-throughput screening approach identified a selective pro-caspase-10 probe that can inhibit its activity
DescriptionCaspases are cysteine proteases that play a critical role in inflammation and apoptosis. Caspases have a functional role in either the intrinsic apoptotic pathway as initiators of apoptosis or in the extrinsic apoptotic pathway as executioners of apoptosis. Caspase-10 has been speculated to play a principal role as an initiator of apoptosis but the absence of adequate tools, i.e. selective probes, have hindered our understanding of this enzyme as well as the other caspases. Here, we engineered a tobacco etch virus (TEV)-cleavable construct that is compatible with high-throughput screening to identify selective caspase-10 inhibitors. We identified a set of promiscuous caspase inhibitors, including pifithrin-ยต (PFT), an inhibitor of p53 transcriptional activity, as well as promising selective pro-caspase-10 and TEV protease inhibitors.
HostingRepositoryPRIDE
AnnounceDate2025-02-04
AnnouncementXMLSubmission_2025-02-03_19:53:10.737.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJose Castellon
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-23 15:32:18ID requested
12025-02-03 19:53:11announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: caspase-10,HTS, biotin azide
Contact List
Dr. Keriann M. Backus
contact affiliationAssistant Professor, Chemistry and Biochemistry, Biological Chemistry Member, Biochemistry, Biophysics & Structural Biology GPB Home Area (lab head)
contact emailKBackus@mednet.ucla.edu
lab head
Jose Castellon
contact affiliationUCLA
contact emailjoseomar805@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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