PXD053308 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted dephosphorylation of TFEB promotes its nuclear translocation |
Description | Reversible phosphorylation of the transcription factor EB (TFEB) coordinates cellular responses to metabolic and other stresses. During nutrient replete and stressor-free conditions, phosphorylated TFEB is primarily localised to the cytoplasm. Stressor-mediated reduction of TFEB phosphorylation promotes its nuclear translocation and context-dependent transcriptional activity. In this study, we explored targeted dephosphorylation of TFEB as an approach to activate TFEB in the absence of nutrient deprivation or other cellular stress. Through an induction of proximity between TFEB and several phosphatases using the AdPhosphatase system, we demonstrate targeted dephosphorylation of TFEB in cells. Furthermore, by developing a heterobifunctional molecule BDPIC (bromoTAG-dTAG proximity-inducing chimera), we demonstrate targeted dephosphorylation of TFEB-dTAG through induced proximity to bromoTAG-PPP2CA. Targeted dephosphorylation of TFEB-dTAG by bromoTAG-PPP2CA with BDPIC at the endogenous levels is sufficient to induce nuclear translocation and some transcriptional activity of TFEB. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:53:38.457.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gopal Sapkota |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-22 21:06:42 | ID requested | |
1 | 2024-08-09 03:21:04 | announced | |
⏵ 2 | 2024-10-22 06:53:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.isci.2024.110432; |
Zhao JF, Shpiro N, Sathe G, Brewer A, Macartney TJ, Wood NT, Negoita F, Sakamoto K, Sapkota GP, Targeted dephosphorylation of TFEB promotes its nuclear translocation. iScience, 27(8):110432(2024) [pubmed] |
Keyword List
submitter keyword: BDPIC, PPP2CA,TFEB, AdPhosphatase, PHOSTAC |
Contact List
Gopal Sapkota |
contact affiliation | Dr Prof .Gopal Sapkota, MRC PPU, School of Life Sciences University of Dundee (lab head) |
contact email | g.sapkota@dundee.ac.uk |
lab head | |
Gopal Sapkota |
contact affiliation | University of Dundee |
contact email | g.sapkota@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053308
- Label: PRIDE project
- Name: Targeted dephosphorylation of TFEB promotes its nuclear translocation