PXD053289

PXD053289 is an original dataset announced via ProteomeXchange.

Title	Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum
Description	The present work developed a non-affinity-based chromatographic method to enrich MUC16 from serum. The enriched MUC16 sample was further processed using a Midi Top 14 abundant protein depletion column. Peptides identified using bottom-up proteomics yielded 1–8% coverage of MUC16. Additionally, MUC16 was detected in samples containing less than the clinical cut-off level of CA125 (35 U/mL), suggesting that this strategy of enrichment and bottom-up proteomics can enable analysis of CA125 from serum of individuals with early-stage ovarian cancer and those whose tumors express CA125 (MUC16) at low levels.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:40:19.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebecca Whelan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-21 16:36:37	ID requested
⏵ 1	2024-10-17 02:40:19	announced

10.1039/d4ay01172d;

Schuster-Little N, Sokolovsky AD, Gentry A, Saraf A, Etzel MR, Patankar MS, Whelan RJ, Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum enables mass spectrometry characterization. Anal Methods, 16(37):6337-6348(2024) [pubmed]

submitter keyword: Human, plasma, ovarian, MUC16, CA125

Rebecca Whelan
contact affiliation	Department of Chemistry, University of Kansas
contact email	rwhelan1@ku.edu
lab head
Rebecca Whelan
contact affiliation	Department of Chemistry, University of Kansas
contact email	rwhelan1@ku.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053289

PRIDE project URI

• PRIDE
  1. PXD053289
     1. Label: PRIDE project
     2. Name: Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum