PXD053289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum |
Description | The present work developed a non-affinity-based chromatographic method to enrich MUC16 from serum. The enriched MUC16 sample was further processed using a Midi Top 14 abundant protein depletion column. Peptides identified using bottom-up proteomics yielded 1–8% coverage of MUC16. Additionally, MUC16 was detected in samples containing less than the clinical cut-off level of CA125 (35 U/mL), suggesting that this strategy of enrichment and bottom-up proteomics can enable analysis of CA125 from serum of individuals with early-stage ovarian cancer and those whose tumors express CA125 (MUC16) at low levels. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_02:40:19.037.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rebecca Whelan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-21 16:36:37 | ID requested | |
⏵ 1 | 2024-10-17 02:40:19 | announced | |
Publication List
10.1039/d4ay01172d; |
Schuster-Little N, Sokolovsky AD, Gentry A, Saraf A, Etzel MR, Patankar MS, Whelan RJ, Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum enables mass spectrometry characterization. Anal Methods, 16(37):6337-6348(2024) [pubmed] |
Keyword List
submitter keyword: Human, plasma, ovarian, MUC16, CA125 |
Contact List
Rebecca Whelan |
contact affiliation | Department of Chemistry, University of Kansas |
contact email | rwhelan1@ku.edu |
lab head | |
Rebecca Whelan |
contact affiliation | Department of Chemistry, University of Kansas |
contact email | rwhelan1@ku.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053289 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053289
- Label: PRIDE project
- Name: Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum