PXD053273

PXD053273 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of cobalamin-dependent propionate production by the rumen bacterium Xylanibacter ruminicola
Description	Xylanibacter ruminicola is an abundant rumen bacterium that produces propionate in a cobalamin-dependent manner. However, the effect of cobalamin supplementation on the expression of propionate pathway enzymes has yet to be investigated. To assess this, comparative transcriptomics and proteomics was carried out on X. ruminicola KHP1 cultures grown in defined media in the presence and absence of cobalamin. Cobalamin supplementation did not affect expression of most propionate pathway proteins, with the only differentially abundant pathway enzyme being the cobalamin-dependent methylmalonyl-CoA mutase, which showed a minor upregulation in the presence of cobalamin. Our results demonstrate expression of the propionate pathway regardless of cobalamin availability, suggesting that cobalamin-dependent induction of propionate production by X. ruminicola is controlled post-translationally.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:37.094.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sam Mahoney-Kurpe
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Xylanibacter ruminicola; NCBI TaxID: 839;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-20 23:58:14	ID requested
1	2024-10-15 01:50:07	announced
⏵ 2	2024-10-22 07:01:37	announced	2024-10-22: Updated project metadata.

10.1128/MSYSTEMS.00864-24;

submitter keyword: propionate,Rumen bacteria, Xylanibacter ruminicola

Dr Christina D. Moon
contact affiliation	AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand.
contact email	christina.moon@agresearch.co.nz
lab head
Sam Mahoney-Kurpe
contact affiliation	AgResearch Ltd, Grasslands Research Centre
contact email	sam.mahoneykurpe@agresearch.co.nz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053273

PRIDE project URI

• PRIDE
  1. PXD053273
     1. Label: PRIDE project
     2. Name: Comparative proteomics of cobalamin-dependent propionate production by the rumen bacterium Xylanibacter ruminicola