PXD053266
PXD053266 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Epigenetic landscape remodeled by the transcription factor RBPJ promotes tumor-infiltrating T cell exhaustion_HUWE1 |
| Description | The suppression of PDL1 expression through inhibition of the assembly of the RBPJ transcription complex has been shown to alleviate T cell exhaustion. However, the specific role of RBPJ in T cells, particularly its potential involvement in the anti-tumor activity of T cells, remains largely unexplored. In this research, immunoprecipitation was employed to identify HUWE1 as the E3 ubiquitin ligase for RBPJ. The integration of CUT&Tag, ChIP/ATAC-qPCR, immunoprecipitation, and flow cytometry analysis revealed that RBPJ induced T cell dysfunction through alterations in the epigenetic landscape, enhancement of transcriptional activity, and modulation of histone methylation/acetylation on exhaustion genes. Additionally, virtual screening and molecular dynamics simulation were utilized to demonstrate that Acarbose disrupted the function of RBPJ by reshaping the assembly of the RBPJ-RUVBL1/DNA ternary complex. Our findings elucidated the induction of RBPJ expression in exhausted T cells and the exacerbation of T cell exhaustion by RBPJ. Additionally, the molecular conformational changes of the RBPJ transcriptional complex induced by Acarbose shed light on the potential of targeted inhibition of T cell exhaustion for the treatment of hepatocellular carcinoma. |
| HostingRepository | iProX |
| AnnounceDate | 2024-06-20 |
| AnnouncementXML | Submission_2024-06-20_21:45:33.715.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Banglun Pan |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-20 21:44:15 | ID requested | |
| ⏵ 1 | 2024-06-20 21:45:34 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Acarbose, HUWE1, RBPJ, T cell exhaustion, Ubiquitination |
Contact List
| Banglun Pan | |
|---|---|
| contact affiliation | Fujian Medical University |
| contact email | 1335563838@qq.com |
| lab head | |
| Banglun Pan | |
| contact affiliation | Fujian Medical University |
| contact email | 1335563838@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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