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PXD053265

PXD053265 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis of proteins co-immunoprecipitated with MIDN-Flag in PC-12 cells
Description We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We performed LC-MS/MS analysis of flag-immunoprecipitated samples from the nuclear fraction of PC-12 cells expressing flag-tagged MIDN (MIDN-Flag) or pcDNA3.1(+) (the empty vector) treated with MG132 (5 μM, 6 h) and nerve growth factor (500 ng/mL, 2 h).
HostingRepositoryjPOST
AnnounceDate2024-09-13
AnnouncementXMLSubmission_2024-09-13_02:36:58.876.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAyano Chiba
SpeciesList scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-20 20:08:41ID requested
12024-09-13 02:36:59announced
Publication List
Chiba A, Kato C, Nakagawa T, Osaki T, Nakamura K, Norota I, Nagashima M, Hosoi T, Ishii K, Obara Y, Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells. Mol Cell Biol, 44(11):516-527(2024) [pubmed]
Keyword List
submitter keyword: PC-12, nerve growth factor
Contact List
Yutaro Obara
lab head
Ayano Chiba
contact affiliationDepartment of Pharmacy, Yamagata University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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