PXD053265

PXD053265 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics analysis of proteins co-immunoprecipitated with MIDN-Flag in PC-12 cells
Description	We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We performed LC-MS/MS analysis of flag-immunoprecipitated samples from the nuclear fraction of PC-12 cells expressing flag-tagged MIDN (MIDN-Flag) or pcDNA3.1(+) (the empty vector) treated with MG132 (5 μM, 6 h) and nerve growth factor (500 ng/mL, 2 h).
HostingRepository	jPOST
AnnounceDate	2024-09-13
AnnouncementXML	Submission_2024-09-13_02:36:58.876.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ayano Chiba
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-20 20:08:41	ID requested
⏵ 1	2024-09-13 02:36:59	announced

Publication List

Chiba A, Kato C, Nakagawa T, Osaki T, Nakamura K, Norota I, Nagashima M, Hosoi T, Ishii K, Obara Y, Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells. Mol Cell Biol, 44(11):516-527(2024) [pubmed]

Chiba A, Kato C, Nakagawa T, Osaki T, Nakamura K, Norota I, Nagashima M, Hosoi T, Ishii K, Obara Y, Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells. Mol Cell Biol, 44(11):516-527(2024) [pubmed]

Keyword List

submitter keyword: PC-12, nerve growth factor

submitter keyword: PC-12, nerve growth factor

Contact List

Yutaro Obara
lab head
Ayano Chiba
contact affiliation	Department of Pharmacy, Yamagata University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003179/