PXD053262

PXD053262 is an original dataset announced via ProteomeXchange.

Title	8-Oxoguanine DNA glycosylase 1 conceals guanine oxidation in nucleoprotein-associated RNA of respiratory syncytial virus
Description	Respiratory syncytial virus (RSV), along with other prominent respiratory RNA viruses such as influenza and SARS-CoV-2, significantly contributes to the global incidence of respiratory tract infections. These pathogens induce the production of reactive oxygen species (ROS), which play a crucial role in the onset and progression of respiratory diseases. However, the mechanisms by which viral RNA manages ROS-induced base oxidation remain poorly understood. Here we reveal that 8-oxo-7,8-dihydroguanine (8-oxoGua) is not merely an incidental byproduct of ROS activity but serves as a strategic adaptation of RSV RNA to maintain genetic fidelity within host cells. Through 8-oxoguanine DNA glycosylase 1 (OGG1) immunoprecipitation and LC_MS/MS, we discovered that RSV nucleoprotein is a principal partner of OGG1. Further investigation revealed that viral ribonucleoprotein complexes specifically hijack OGG1. Importantly, inhibiting OGG1's ability to recognize 8-oxoGua significantly reduces RSV progeny production. Our results underscore the viral replication machinery's adaptation to oxidative challenges, suggesting that inhibiting OGG1's reading function could be a novel strategy for antiviral intervention.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:54.926.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053262
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lee Palmer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human respiratory syncytial virus A; NCBI TaxID: 208893;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-20 14:04:18	ID requested
1	2024-10-09 02:18:20	announced
⏵ 2	2024-10-22 07:00:55	announced	2024-10-22: Updated project metadata.

10.6019/PXD053262;

submitter keyword: Oxidative stress, OGG1, 8-oxoguanine, RNA oxidation

William K. Russell
contact affiliation	Associate Professor, Department of Biochemistry & Molecular Biology, UTMB Mass Spectrometry Director, University of Texas Medical Branch – Galveston, Texas, USA
contact email	wirussel@utmb.edu
lab head
Lee Palmer
contact affiliation	University of Texas Medical Branch at Galveston
contact email	lkpalmer@utmb.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053262
     1. Label: PRIDE project
     2. Name: 8-Oxoguanine DNA glycosylase 1 conceals guanine oxidation in nucleoprotein-associated RNA of respiratory syncytial virus