PXD053251

PXD053251 is an original dataset announced via ProteomeXchange.

Title	Trypanosomal Pam18/Pam16 and maxicircle replication – MaRF11 SILAC RNAi
Description	Protein import and genome replication are essential processes for mitochondrial biogenesis and propagation. The J-domain proteins Pam16 and Pam18 regulate the presequence translocase of the mitochondrial inner membrane. In the protozoan Trypanosoma brucei, their counterparts are TbPam16 and TbPam18, which are essential for the procyclic form of the parasite, though not involved in mitochondrial protein import. Here, we show that during evolution, the two proteins have been repurposed to regulate the replication of maxicircles within the intricate kDNA network, the most complex mitochondrial genome known. TbPam18 and TbPam16 have inactive J-domains suggesting a function independent of heat shock proteins. However, their single transmembrane domain is essential for function. Pulldown of TbPam16 identifies a putative client protein, termed MaRF11, the depletion of which causes the selective loss of maxicircles, akin to the effects observed for TbPam18 and TbPam16. The level of MaRF11 is controlled by the mitochondrial proteasome. Thus, we propose a model for a membrane-bound regulatory circuit that controls maxicircle replication in response to an unknown nuclear signal. This model posits that MaRF11 directly mediates maxicircle replication, that its activity is regulated by the mitochondrial proteasome that its level is controlled by proteasomal digested that it is protected from degradation by binding to the TbPam18/TbPam16 dimer.
HostingRepository	PRIDE
AnnounceDate	2024-07-20
AnnouncementXML	Submission_2024-07-20_07:22:17.348.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Bender
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-20 07:34:37	ID requested
⏵ 1	2024-07-20 07:22:17	announced

Dataset with its publication pending

submitter keyword: Trypanosoma brucei, proteomics, mitochondrial genome replication,SILAC, maxicircle replication, RNAi-mediated protein knockdown

Bettina Warscheid
contact affiliation	Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany
contact email	bettina.warscheid@uni-wuerzburg.de
lab head
Julian Bender
contact affiliation	University of Wuerzburg Chair of Biochemistry II Am Hubland 97074 Würzburg
contact email	julian.bender@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053251
     1. Label: PRIDE project
     2. Name: Trypanosomal Pam18/Pam16 and maxicircle replication – MaRF11 SILAC RNAi