PXD053246

PXD053246 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	human LHR-APEX2 time-resolved proximity proteomics
Description	Cellular signaling by membrane G protein-coupled receptors (GPCRs) is orchestrated by a complex and diverse array of mechanisms. The dynamics of a GPCR interactome as it evolves over time and space in response to an agonist can offer a unique window on pleiotropic signaling decoding and functional selectivity at a cellular level. In this study, we employed proximity-based APEX2 proteomics to interrogate the interaction network of the GPCR for luteinizing hormone (LHR) on a sub-minute timescale. We developed an analytical approach integrating quantitative multiplexed proteomics and temporal reference profiles, providing a platform to identify the proteomic environment of APEX2-tagged LHR at the nanometer scale. LHR activity is exquisitely regulated at a spatial level, leading to identification of novel interactors including the Ras-related GTPase RAP2B that modulate both receptor signaling and post-endocytic trafficking, and providing a resource for spatiotemporal nanodomain mapping of LHR interactors across subcellular compartments.
HostingRepository	PRIDE
AnnounceDate	2026-03-20
AnnouncementXML	Submission_2026-03-20_05:24:30.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack Houghton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-20 02:22:00	ID requested
⏵ 1	2026-03-20 05:24:31	announced

Publication List

10.1016/j.chembiol.2025.04.006;
Shchepinova MM, Richardson R, Houghton JW, Walker AR, Safar MA, Conole D, Hanyaloglu AC, Tate EW, Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism. Cell Chem Biol, 32(5):722-735.e7(2025) [pubmed]

10.1016/j.chembiol.2025.04.006;

Shchepinova MM, Richardson R, Houghton JW, Walker AR, Safar MA, Conole D, Hanyaloglu AC, Tate EW, Spatiotemporally resolved GPCR interactome uncovers unique mediators of receptor agonism. Cell Chem Biol, 32(5):722-735.e7(2025) [pubmed]

Keyword List

submitter keyword: proteomics, proximity proteomics, APEX2, LHCGR, LHR, interactomics, GPCR,human

submitter keyword: proteomics, proximity proteomics, APEX2, LHCGR, LHR, interactomics, GPCR,human

Contact List

Edward Tate
contact affiliation	Imperial College London
contact email	e.tate@imperial.ac.uk
lab head
Jack Houghton
contact affiliation	Imperial College London
contact email	j.houghton@imperial.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD053246
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD053246

PRIDE project URI

Repository Record List

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