PXD053209

PXD053209 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Unique structural features of the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex
Description	The human NuA4/TIP60 co-activator complex, a fusion of the yeast SWR1 and NuA4 complexes, both incorporates the histone variant H2A.Z into nucleosomes and acetylates histones H4/H2A/H2A.Z to play crucial roles regulating gene expression and maintaining genome stability. Our cryo-EM studies show that within the NuA4/TIP60 complex, the EP400 subunit serves as an architectural scaffold holding the different functional modules in specific positions and giving rise to a novel arrangement of the ARP module. EP400 interacts with the TRRAP subunit using a footprint that overlaps with that of the SAGA acetyltransferase complex, thereby preventing the formation of a hybrid complex. Loss of the TRRAP subunit leads to mislocalization of NuA4/TIP60, resulting in the redistribution of H2A.Z and its acetylation across the genome, emphasizing the dual functionality of NuA4/TIP60 as a single macromolecular assembly.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:52:39.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Luo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-18 10:18:00	ID requested
1	2024-08-08 02:22:36	announced
⏵ 2	2024-10-22 06:52:40	announced	2024-10-22: Updated project metadata.

Publication List

10.1126/science.adl5816;
Yang Z, Mameri A, Cattoglio C, Lachance C, Florez Ariza AJ, Luo J, Humbert J, Sudarshan D, Banerjea A, Galloy M, Fradet-Turcotte A, Lambert JP, Ranish JA, C, ô, t, é J, Nogales E, Structural insights into the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex. Science, 385(6711):eadl5816(2024) [pubmed]

10.1126/science.adl5816;

Yang Z, Mameri A, Cattoglio C, Lachance C, Florez Ariza AJ, Luo J, Humbert J, Sudarshan D, Banerjea A, Galloy M, Fradet-Turcotte A, Lambert JP, Ranish JA, C, ô, t, é J, Nogales E, Structural insights into the human NuA4/TIP60 acetyltransferase and chromatin remodeling complex. Science, 385(6711):eadl5816(2024) [pubmed]

Keyword List

submitter keyword: NuA4/TIP60 acetyltransferase, P400-NCP complex, H2A.Z,NuA4 and SWR1 chromatin modifier and remodeler complexes

submitter keyword: NuA4/TIP60 acetyltransferase, P400-NCP complex, H2A.Z,NuA4 and SWR1 chromatin modifier and remodeler complexes

Contact List

Jeff Ranish
contact affiliation	Institute for Systems Biology
contact email	jeff.ranish@isbsciecne.org
lab head
Jie Luo
contact affiliation	Institute for Systems Biology
contact email	jluo@systemsbiology.org
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD053209
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD053209

PRIDE project URI

Repository Record List

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