PXD053204
PXD053204 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells. |
| Description | Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe-derived RNAs carried by outer membrane vesicles (OMVs) to host immune responses remain unclear. This study investigated the role of OMVs and OMV-associated small RNA (sRNA) species from pathogenic and commensal Bacteroides fragilis (ETBF and NTBF respectively) in eliciting different immune responses from intestinal epithelial cells. To distinguish the differences in the sRNA profiles of the two strains and their OMVs, RNA-seq, qRT-PCR, and northern blotting were conducted to identify enrichment of discrete sRNA species in OMVs, which were also differentially expressed between the two strains. To understand the effects of OMVs on pattern recognition receptors, Reporter cells of Toll-like receptor (TLR) activation were treated with OMVs, demonstrating activation of TLRs 2, 3, and 7. Treatment of Caco-2 and HT29-MTX cells with OMVs demonstrated increased expression of IL-8. Surprisingly, we discovered that degradation of RNase-accessible RNAs within ETBF OMVs, but not NTBF OMVs, resulted in vesicles with enhanced capacity to stimulate IL-8 expression, indicating that these extravesicular RNAs exert an immunosuppressive effect. This suggests a dual role for OMV-associated RNAs in modulating host immune responses, with implications for both bacterial pathogenesis and therapeutic applications. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-23 |
| AnnouncementXML | Submission_2024-11-23_11:34:17.410.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jessica Conforti |
| SpeciesList | scientific name: Bacteroides fragilis; NCBI TaxID: 817; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Synapt G2-S HDMS; nanoACQUITY UPLC |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-18 07:48:55 | ID requested | |
| ⏵ 1 | 2024-11-23 11:34:17 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Bacteroides Fragilis, ETBF, NTBF |
Contact List
| Elyssia Gallagher | |
|---|---|
| contact affiliation | Baylor University |
| contact email | elyssia_gallagher@baylor.edu |
| lab head | |
| Jessica Conforti | |
| contact affiliation | Baylor University |
| contact email | jess_conforti1@baylor.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095065/ |
to receive all new ProteomeXchange dataset release announcements!
