PXD053176

PXD053176 is an original dataset announced via ProteomeXchange.

Title	Mytilus galloprovincialis cryopreserved oocytes LC-MS/MS data
Description	Cryopreservation of gametes offers significant potential for genetic diversity conservation and selective breeding in aquaculture. The blue mussel, Mytilus galloprovincialis, is valuable in the aquaculture industry, but declining seed availability and the need for efficient hatchery production, especially outside its natural reproductive season, make gamete cryopreservation essential. However, cryopreserving gametes, particularly oocytes from marine invertebrates, is challenging due to the balance required between cryoprotectant cytotoxicity and cell protection during freezing-thawing. This study investigates the effects of two common cryoprotectants, dimethyl sulfoxide (DMSO) and ethylene glycol (EG), on M. galloprovincialis oocytes using a monitored slow freezing (MSF) protocol. Our integrative analytical approach combined proteomics, functional, and ultrastructural analyses. Findings highlight that cryoprotectants induce significant proteomic alterations, more pronounced with DMSO than EG. These alterations are linked to oxidative stress, ineffective antioxidant response, and disruptions in meiosis restart mechanisms, leading to delayed larvae development. The MSF protocol resulted in plasma membrane rupture and lack of fertilisation success post-thawing. Our results provide the first report on the molecular basis underlying the limited success in cryopreserving mussel oocytes, showing EG as less harmful compared to DMSO, and supports supplementing cryoprotectants with external antioxidants and membrane stabilisers to improve cryopreservation of marine invertebrate oocytes.
HostingRepository	PRIDE
AnnounceDate	2025-08-29
AnnouncementXML	Submission_2025-08-29_09:27:39.722.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053176
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sofía Blanco
SpeciesList	scientific name: Mytilus galloprovincialis (Mediterranean mussel); NCBI TaxID: 29158;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-17 10:55:45	ID requested
⏵ 1	2025-08-29 09:27:40	announced

10.6019/PXD053176;

10.1016/J.CRYOBIOL.2025.105288;

submitter keyword: cryoprotectant agent, in vitro fertilisation, oxidative stress,Mytilus galloprovincialis, cytotoxicity

Ángel P. Diz
contact affiliation	Department of Biochemistry, Genetics and Immunology, University of Vigo, Spain
contact email	angel.p.diz@uvigo.es
lab head
Sofía Blanco
contact affiliation	University of Vigo
contact email	sofiablancoglez@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053176
     1. Label: PRIDE project
     2. Name: Mytilus galloprovincialis cryopreserved oocytes LC-MS/MS data