PXD053174

PXD053174 is an original dataset announced via ProteomeXchange.

Title	A novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N labeled proteins
Description	Recent developments in affinity binder or mass spectrometry (MS)-based plasma proteomics are now producing panels of potential biomarker candidates for diagnosis or prognosis. However, clinical validation and implementation of these biomarkers remain limited by the reliance on dated triple quadrupole MS technology. Here, we evaluate a novel hybrid high-speed mass spectrometer, Stellar MS, which integrates the robustness of triple quadrupoles with the enhanced capabilities of an advanced linear ion trap detector. This instrument allows for extremely rapid and sensitive parallel reaction monitoring (PRM) and MS3 targeting. We successfully targeted thousands of peptides measured on Orbitrap Astral MS on the Stellar MS, achieving high reproducibility and low coefficients of variation (CV) as well as sensitivity and specificity sufficient for more than the top 1000 plasma proteins. Furthermore, we developed targeted assays for alcohol-related liver disease (ALD) biomarkers, showcasing the potential of Stellar MS in clinical applications. Absolute quantification is typically a requirement for clinical assays and we explore the use of 15N labeled protein standards in a rapid, streamlined and generic manner. Our results indicate that Stellar MS can bridge the gap between proteomics discovery and routine clinical testing, enhancing the diagnostic and prognostic utility of protein biomarkers.
HostingRepository	MassIVE
AnnounceDate	2025-09-24
AnnouncementXML	Submission_2025-09-24_09:32:19.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	mario oroshi
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral; Stellar; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-17 08:40:17	ID requested
⏵ 1	2025-09-24 09:32:20	announced

Wahle M, Remes PM, Albrecht V, Baggio Lorenz M, Mueller-Reif J, Steigerwald S, Heymann T, Niu L, L, ö, ssl P, Horning S, Jacob CC, Mann M, N-Labeled Proteins. Mol Cell Proteomics, 24(9):101050(2025) [pubmed]

submitter keyword: plasma, targeted Proteomics, 15N labeling

Matthias Mann
contact affiliation	Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 D-82152 Martinsried
contact email	mmann@biochem.mpg.de
lab head
mario oroshi
contact affiliation	MPI
contact email	oroshi@biochem.mpg.de
dataset submitter

MassIVE dataset URI

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