PXD053117 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein Extraction methods suitable for muscle tissue proteomic analysis |
Description | • Evaluation of two methods for protein extraction from muscle tissues. • Two different extraction methods showed distinct patterns of protein abundance; • Two-dimensional electrophoresis revealed 131 spots with different volumes; • SDS-based buffer (method A) mostly extracted proteins of high MW range and peculiar in sarcomere and muscular fibres, such as Troponin, Myosin, Miozenin 2 and Ankyrin; • Urea/chaps/DTE/tris solution (method B) enriched numerous protein species in a low MW range, several protein fragments and organelle membrane proteins |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-06_19:35:37.173.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lorenza Vantaggiato |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | ultrafleXtreme |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-14 04:46:34 | ID requested | |
⏵ 1 | 2025-05-06 19:35:38 | announced | |
Publication List
Vantaggiato L, Landi C, Shaba E, Rossi D, Sorrentino V, Bini L, Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis. Proteomes, 12(4):(2024) [pubmed] |
10.3390/proteomes12040027; |
Keyword List
submitter keyword: mass spectrometry,protein denaturation, muscle tissue, two-dimensional electrophoresis, myopathies. |
Contact List
LUCA BINI |
contact affiliation | Functional Proteomics Lab., Dept Life Sciences, University of Siena |
contact email | luca.bini@unisi.it |
lab head | |
Lorenza Vantaggiato |
contact affiliation | University of Siena |
contact email | lorenz.vantaggiato2@unisi.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053117 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053117
- Label: PRIDE project
- Name: Protein Extraction methods suitable for muscle tissue proteomic analysis