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PXD053117

PXD053117 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein Extraction methods suitable for muscle tissue proteomic analysis
Description• Evaluation of two methods for protein extraction from muscle tissues. • Two different extraction methods showed distinct patterns of protein abundance; • Two-dimensional electrophoresis revealed 131 spots with different volumes; • SDS-based buffer (method A) mostly extracted proteins of high MW range and peculiar in sarcomere and muscular fibres, such as Troponin, Myosin, Miozenin 2 and Ankyrin; • Urea/chaps/DTE/tris solution (method B) enriched numerous protein species in a low MW range, several protein fragments and organelle membrane proteins
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_19:35:37.173.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLorenza Vantaggiato
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentultrafleXtreme
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-14 04:46:34ID requested
12025-05-06 19:35:38announced
Publication List
Vantaggiato L, Landi C, Shaba E, Rossi D, Sorrentino V, Bini L, Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis. Proteomes, 12(4):(2024) [pubmed]
10.3390/proteomes12040027;
Keyword List
submitter keyword: mass spectrometry,protein denaturation, muscle tissue, two-dimensional electrophoresis, myopathies.
Contact List
LUCA BINI
contact affiliationFunctional Proteomics Lab., Dept Life Sciences, University of Siena
contact emailluca.bini@unisi.it
lab head
Lorenza Vantaggiato
contact affiliationUniversity of Siena
contact emaillorenz.vantaggiato2@unisi.it
dataset submitter
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Dataset FTP location
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