PXD053117

PXD053117 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein Extraction methods suitable for muscle tissue proteomic analysis
Description	• Evaluation of two methods for protein extraction from muscle tissues. • Two different extraction methods showed distinct patterns of protein abundance; • Two-dimensional electrophoresis revealed 131 spots with different volumes; • SDS-based buffer (method A) mostly extracted proteins of high MW range and peculiar in sarcomere and muscular fibres, such as Troponin, Myosin, Miozenin 2 and Ankyrin; • Urea/chaps/DTE/tris solution (method B) enriched numerous protein species in a low MW range, several protein fragments and organelle membrane proteins
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_19:35:37.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lorenza Vantaggiato
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	ultrafleXtreme

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 04:46:34	ID requested
⏵ 1	2025-05-06 19:35:38	announced

Publication List

Vantaggiato L, Landi C, Shaba E, Rossi D, Sorrentino V, Bini L, Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis. Proteomes, 12(4):(2024) [pubmed]
10.3390/proteomes12040027;

Vantaggiato L, Landi C, Shaba E, Rossi D, Sorrentino V, Bini L, Protein Extraction Methods Suitable for Muscle Tissue Proteomic Analysis. Proteomes, 12(4):(2024) [pubmed]

10.3390/proteomes12040027;

Keyword List

submitter keyword: mass spectrometry,protein denaturation, muscle tissue, two-dimensional electrophoresis, myopathies.

submitter keyword: mass spectrometry,protein denaturation, muscle tissue, two-dimensional electrophoresis, myopathies.

Contact List

LUCA BINI
contact affiliation	Functional Proteomics Lab., Dept Life Sciences, University of Siena
contact email	luca.bini@unisi.it
lab head
Lorenza Vantaggiato
contact affiliation	University of Siena
contact email	lorenz.vantaggiato2@unisi.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053117
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053117

PRIDE project URI

Repository Record List

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