PXD053105 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cellular response to incubation with pristine carbon nanodots |
Description | The cellular response of MCF-7 cells after uptake of pristine carbon nanodots (CNDs) was investigated by MS-based proteomics and secretomics. Only minor changes in the proteomes and secretomes were observed independetly from the medium (full medium: RPMI1640 with FBS/PS/L-Glu, basal medium: RPMI1640 without additives, or starvation medium: Earle's Balanced Salt Solution (EBSS)). Further investigations showed that even under high CND concentrations, the lysosomal functionality, as characterized via cathepsins B and L as well as the autophagic markers SQSTM1/p62 and LC3B, is maintained. Furthermore, branched polyethylenimine (bPEI) molecules have been coupled to the CNDs as a model functionalization or example of a drug. We observed that the bPEI-CND conjugates accumulate to a higher degree in the lysosomes as compared to bPEI or CND alone. Here, changes in the lysosomal size and function were observed, which can be explained exclusively by the bPEI. It is concluded that CNDs are highly efficient and inert carriers for functional molecules into lysosomes as target, with the added value that lysosomal escape is suppressed, thereby avoiding unintended side effects in other cellular compartments. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-03 |
AnnouncementXML | Submission_2025-02-03_11:45:25.402.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Lenz |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-14 03:13:19 | ID requested | |
⏵ 1 | 2025-02-03 11:45:25 | announced | |
Publication List
Keyword List
submitter keyword: carbon nanodots, proteomics, secretomics, CNDs, inert drug carriers, lysosomes, MCF-7 cells |
Contact List
Kai Stühler |
contact affiliation | MPL/BMFZ, HHU Duesseldorf |
contact email | kai.stuehler@hhu.de |
lab head | |
Thomas Lenz |
contact affiliation | MPL/BMFZ, HHU Düsseldorf |
contact email | thomas.lenz@hhu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053105
- Label: PRIDE project
- Name: Cellular response to incubation with pristine carbon nanodots