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PXD053105

PXD053105 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCellular response to incubation with pristine carbon nanodots
DescriptionThe cellular response of MCF-7 cells after uptake of pristine carbon nanodots (CNDs) was investigated by MS-based proteomics and secretomics. Only minor changes in the proteomes and secretomes were observed independetly from the medium (full medium: RPMI1640 with FBS/PS/L-Glu, basal medium: RPMI1640 without additives, or starvation medium: Earle's Balanced Salt Solution (EBSS)). Further investigations showed that even under high CND concentrations, the lysosomal functionality, as characterized via cathepsins B and L as well as the autophagic markers SQSTM1/p62 and LC3B, is maintained. Furthermore, branched polyethylenimine (bPEI) molecules have been coupled to the CNDs as a model functionalization or example of a drug. We observed that the bPEI-CND conjugates accumulate to a higher degree in the lysosomes as compared to bPEI or CND alone. Here, changes in the lysosomal size and function were observed, which can be explained exclusively by the bPEI. It is concluded that CNDs are highly efficient and inert carriers for functional molecules into lysosomes as target, with the added value that lysosomal escape is suppressed, thereby avoiding unintended side effects in other cellular compartments.
HostingRepositoryPRIDE
AnnounceDate2025-02-03
AnnouncementXMLSubmission_2025-02-03_11:45:25.402.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Lenz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-14 03:13:19ID requested
12025-02-03 11:45:25announced
Publication List
10.1016/J.ISCI.2024.111654;
Keyword List
submitter keyword: carbon nanodots, proteomics, secretomics, CNDs, inert drug carriers, lysosomes, MCF-7 cells
Contact List
Kai Stühler
contact affiliationMPL/BMFZ, HHU Duesseldorf
contact emailkai.stuehler@hhu.de
lab head
Thomas Lenz
contact affiliationMPL/BMFZ, HHU Düsseldorf
contact emailthomas.lenz@hhu.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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