PXD053105

PXD053105 is an original dataset announced via ProteomeXchange.

Title	Cellular response to incubation with pristine carbon nanodots
Description	The cellular response of MCF-7 cells after uptake of pristine carbon nanodots (CNDs) was investigated by MS-based proteomics and secretomics. Only minor changes in the proteomes and secretomes were observed independetly from the medium (full medium: RPMI1640 with FBS/PS/L-Glu, basal medium: RPMI1640 without additives, or starvation medium: Earle's Balanced Salt Solution (EBSS)). Further investigations showed that even under high CND concentrations, the lysosomal functionality, as characterized via cathepsins B and L as well as the autophagic markers SQSTM1/p62 and LC3B, is maintained. Furthermore, branched polyethylenimine (bPEI) molecules have been coupled to the CNDs as a model functionalization or example of a drug. We observed that the bPEI-CND conjugates accumulate to a higher degree in the lysosomes as compared to bPEI or CND alone. Here, changes in the lysosomal size and function were observed, which can be explained exclusively by the bPEI. It is concluded that CNDs are highly efficient and inert carriers for functional molecules into lysosomes as target, with the added value that lysosomal escape is suppressed, thereby avoiding unintended side effects in other cellular compartments.
HostingRepository	PRIDE
AnnounceDate	2025-02-03
AnnouncementXML	Submission_2025-02-03_11:45:25.402.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 03:13:19	ID requested
⏵ 1	2025-02-03 11:45:25	announced

10.1016/J.ISCI.2024.111654;

submitter keyword: carbon nanodots, proteomics, secretomics, CNDs, inert drug carriers, lysosomes, MCF-7 cells

Kai Stühler
contact affiliation	MPL/BMFZ, HHU Duesseldorf
contact email	kai.stuehler@hhu.de
lab head
Thomas Lenz
contact affiliation	MPL/BMFZ, HHU Düsseldorf
contact email	thomas.lenz@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053105

PRIDE project URI

• PRIDE
  1. PXD053105
     1. Label: PRIDE project
     2. Name: Cellular response to incubation with pristine carbon nanodots