PXD053103

PXD053103 is an original dataset announced via ProteomeXchange.

Title	Cryo-EM Structures of 30S:mRNA Complexes in Saccharolobus solfataricus: Unveiling Archaeal Features and Eukaryotic Proximity
Description	The archaeal ribosome is of the eukaryotic type. Genomic and phylogenetic studies have indicated that TACK and Asgard, the closest relatives of eukaryotes, have ribosomes containing eukaryotic ribosomal proteins not found in other archaeal branches, eS25, eS26 and eS30. In our study, we investigated the case of Saccharolobus solfataricus, a crenarchaea belonging to the TACK branch, which mainly uses leaderless mRNAs. We characterized the small ribosomal subunit of S. Solfataricus bound to SD-leadered or leaderless mRNAs. Cryo-EM structures show for the first time archaeal versions of the eS25, eS26 and eS30 proteins bound to the small subunit. In addition, we identify two novel ribosomal proteins named aS33 and aS34 as well as a domain of eS6, that highlight the diversity of archaeal ribosomes. Leaderless mRNAs are bound to the small ribosomal subunit and the 5'-triphosphate group contributes to their binding. Archaeal eS26 is in the mRNA exit channel wrapped around the 3' end of ribosomal RNA as it is in eukaryotes. Its position is not compatible with an SD:antiSD duplex in the mRNA exit channel. Overall, our results suggest a role of eS26 in translation regulation and possible evolutionary routes from archaeal to eukaryotic translation.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_19:31:35.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Sulfolobus solfataricus; NCBI TaxID: 2287;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 02:44:56	ID requested
⏵ 1	2025-05-06 19:31:36	announced

Bourgeois G, Coureux PD, Lazennec-Schurdevin C, Madru C, Gaillard T, Duchateau M, Chamot-Rooke J, Bourcier S, Mechulam Y, Schmitt E, Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity. Nat Commun, 16(1):348(2025) [pubmed]

10.1038/s41467-024-55718-5;

submitter keyword: eS26, eS25,translation initiation, eS30, leaderless mRNA

Emmanuelle Schmitt
contact affiliation	Laboratoire de Biologie Structurale de la Cellule, BIOC Ecole polytechnique CNRS Institut Polytechnique de Paris 91128 Palaiseau cedex France
contact email	emmanuelle.schmitt@polytechnique.edu
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053103

PRIDE project URI

• PRIDE
  1. PXD053103
     1. Label: PRIDE project
     2. Name: Cryo-EM Structures of 30S:mRNA Complexes in Saccharolobus solfataricus: Unveiling Archaeal Features and Eukaryotic Proximity