PXD053085

PXD053085 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human colorectal cancer cell lines with manipulated AGR2 expression SWATH MS
Description	SWATH MS analysis of colorectal adenocarcinoma derived paired cellular model, namely SW480 derived from a primary tumour and SW620 derived from the tumour´s lymph node metastasis. The whole proteome spectra were analysed to assess the changes caused by the manipulation of AGR2 expression. AGR2, a protein disulphide isomerase, is often overexpressed in tumours and connected with proliferation, survival, chemoresistance, and metastasis. Parental SW480 is AGR2 negative, so a stable expressing clone was prepared, while SW620 is endogenously positive for AGR2; therefore, two CRISPR/Cas9 mediated knockout clones were generated. The SWATH MS analysis generated a set of differentially expressed proteins with respect to AGR2 expression. This allowed us to identify new potential regulatory mechanisms and molecular pathways in which the AGR2 might be involved.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_03:58:08.956.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakub Faktor
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-13 12:04:36	ID requested
⏵ 1	2025-04-23 03:58:09	announced

Publication List

Martisova A, Faktor J, Sosolikova T, Klemesova I, Kolarova T, Holcakova J, Hrstka R, Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer. Sci Rep, 14(1):21926(2024) [pubmed]
10.1038/s41598-024-72990-z;

Martisova A, Faktor J, Sosolikova T, Klemesova I, Kolarova T, Holcakova J, Hrstka R, Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer. Sci Rep, 14(1):21926(2024) [pubmed]

10.1038/s41598-024-72990-z;

Keyword List

submitter keyword: SW480, colorectal adenocarcinoma, SW620, AGR2, SWATH MS

submitter keyword: SW480, colorectal adenocarcinoma, SW620, AGR2, SWATH MS

Contact List

Lenka Hernychova
contact affiliation	Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty Kopec 7, 65653 Brno, Czech Republic
contact email	lenka.hernychova@mou.cz
lab head
Jakub Faktor
contact affiliation	University of Gdansk Kladki 24 Gdansk
contact email	jakub.faktor@ug.edu.pl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053085
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053085

PRIDE project URI

Repository Record List

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