PXD053085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human colorectal cancer cell lines with manipulated AGR2 expression SWATH MS |
Description | SWATH MS analysis of colorectal adenocarcinoma derived paired cellular model, namely SW480 derived from a primary tumour and SW620 derived from the tumour“s lymph node metastasis. The whole proteome spectra were analysed to assess the changes caused by the manipulation of AGR2 expression. AGR2, a protein disulphide isomerase, is often overexpressed in tumours and connected with proliferation, survival, chemoresistance, and metastasis. Parental SW480 is AGR2 negative, so a stable expressing clone was prepared, while SW620 is endogenously positive for AGR2; therefore, two CRISPR/Cas9 mediated knockout clones were generated. The SWATH MS analysis generated a set of differentially expressed proteins with respect to AGR2 expression. This allowed us to identify new potential regulatory mechanisms and molecular pathways in which the AGR2 might be involved. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-23 |
AnnouncementXML | Submission_2025-04-23_03:58:08.956.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jakub Faktor |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-13 12:04:36 | ID requested | |
⏵ 1 | 2025-04-23 03:58:09 | announced | |
Publication List
Martisova A, Faktor J, Sosolikova T, Klemesova I, Kolarova T, Holcakova J, Hrstka R, Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer. Sci Rep, 14(1):21926(2024) [pubmed] |
10.1038/s41598-024-72990-z; |
Keyword List
submitter keyword: SW480, colorectal adenocarcinoma, SW620, AGR2, SWATH MS |
Contact List
Lenka Hernychova |
contact affiliation | Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty Kopec 7, 65653 Brno, Czech Republic |
contact email | lenka.hernychova@mou.cz |
lab head | |
Jakub Faktor |
contact affiliation | University of Gdansk
Kladki 24
Gdansk |
contact email | jakub.faktor@ug.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053085
- Label: PRIDE project
- Name: Human colorectal cancer cell lines with manipulated AGR2 expression SWATH MS