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PXD053085

PXD053085 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman colorectal cancer cell lines with manipulated AGR2 expression SWATH MS
DescriptionSWATH MS analysis of colorectal adenocarcinoma derived paired cellular model, namely SW480 derived from a primary tumour and SW620 derived from the tumour“s lymph node metastasis. The whole proteome spectra were analysed to assess the changes caused by the manipulation of AGR2 expression. AGR2, a protein disulphide isomerase, is often overexpressed in tumours and connected with proliferation, survival, chemoresistance, and metastasis. Parental SW480 is AGR2 negative, so a stable expressing clone was prepared, while SW620 is endogenously positive for AGR2; therefore, two CRISPR/Cas9 mediated knockout clones were generated. The SWATH MS analysis generated a set of differentially expressed proteins with respect to AGR2 expression. This allowed us to identify new potential regulatory mechanisms and molecular pathways in which the AGR2 might be involved.
HostingRepositoryPRIDE
AnnounceDate2025-04-23
AnnouncementXMLSubmission_2025-04-23_03:58:08.956.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJakub Faktor
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-13 12:04:36ID requested
12025-04-23 03:58:09announced
Publication List
Martisova A, Faktor J, Sosolikova T, Klemesova I, Kolarova T, Holcakova J, Hrstka R, Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer. Sci Rep, 14(1):21926(2024) [pubmed]
10.1038/s41598-024-72990-z;
Keyword List
submitter keyword: SW480, colorectal adenocarcinoma, SW620, AGR2, SWATH MS
Contact List
Lenka Hernychova
contact affiliationResearch Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty Kopec 7, 65653 Brno, Czech Republic
contact emaillenka.hernychova@mou.cz
lab head
Jakub Faktor
contact affiliationUniversity of Gdansk Kladki 24 Gdansk
contact emailjakub.faktor@ug.edu.pl
dataset submitter
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Dataset FTP location
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