PXD053065

PXD053065 is an original dataset announced via ProteomeXchange.

Title	Chemosensory function of Varroa gnathosoma: Transcriptomic and proteomic analyses
Description	In this study, we evaluated the role of the gnathosomoa (mouthpart) in chemosensing of the most devastating honey bee parasite, Varroa destructor mite. Through transcriptomic analysis, we compared the expression of putative chemosensory genes between the body parts containing the main chemosensory organs (the forelegs), gnathosoma and the rest of the body devoid of these two body parts. Furthermore, we checked the presence of chemosensory-related transcripts in the proteome of the gnathosoma. Our comparative transcriptomic analysis revealed the presence of 83 transcripts with known characteristic conserved domains belonging to eight chemosensory gene families in the three Varroa transcriptomes. Among these transcripts, 11 were significantly upregulated in the mite’s forelegs, compared to 8 and 10 in the gnathosoma and body devoid of both organs, respectively. Whilst the gnathosoma and the forelegs share similar expression of some putative lipid carrier proteins, membrane-bound receptors, and associated proteins, they also differ in the expression profiles of some transcripts belonging to these protein families. This suggests two functional chemosensory organs that may differ in their chemosensory function according to specific characteristics of compounds they detect. Moreover, the higher expression of some chemosensory transcripts in the body devoid of forelegs and gnathosoma compared to the gnathosoma alone, may suggest the presence of additional function of these transcripts or alternatively presence of additional external or internal chemosensory organs. Insights into the functional annotation of a highly expressed gustatory receptor present in both organs using RNA interference (RNAi) are also revealed.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_19:20:52.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nurit Eliash
SpeciesList	scientific name: Varroa destructor; NCBI TaxID: 109461;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-13 01:39:34	ID requested
⏵ 1	2025-05-06 19:20:53	announced

Nganso BT, Eliash N, Mani K, Sela N, Villar-Briones A, Osabutey AF, Rafaeli A, Mikheyev AS, Soroker V, Chemosensory function of Varroa gnathosoma: transcriptomic and proteomic analyses. Exp Appl Acarol, 93(4):701-719(2024) [pubmed]

10.1007/s10493-024-00952-1;

submitter keyword: varroa, gnathosoma, chemosensation

Victoria Soroker
contact affiliation	nstitute of Plant Protection, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel
contact email	sorokerv@volcani.agri.gov.il
lab head
Nurit Eliash
contact affiliation	Shamir research institute, Haifa university, Okinawa Institute of Science and Technology
contact email	nurite@gri.org.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053065
     1. Label: PRIDE project
     2. Name: Chemosensory function of Varroa gnathosoma: Transcriptomic and proteomic analyses